Document Detail


Myeloperoxidase activity is increased in gingival crevicular fluid and whole saliva after fixed orthodontic appliance activation.
MedLine Citation:
PMID:  21055602     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: Orthodontic tooth movement uses mechanical forces that result in inflammation in the first days. Myeloperoxidase (MPO) is an enzyme found in polymorphonuclear neutrophil (PMN) granules, and it is used to estimate the number of PMN granules in tissues. So far, MPO has not been used to study the inflammatory alterations after the application of orthodontic tooth movement forces. The aim of this study was to determine MPO activity in the gingival crevicular fluid (GCF) and saliva (whole stimulated saliva) of orthodontic patients at different time points after fixed appliance activation.
METHODS: MPO was determined in the GCF and collected by means of periopaper from the saliva of 14 patients with orthodontic fixed appliances. GCF and saliva samples were collected at baseline, 2 hours, and 7 and 14 days after application of the orthodontic force.
RESULTS: Mean MPO activity was increased in both the GCF and saliva of orthodontic patients at 2 hours after appliance activation (P <0.02 for all comparisons). At 2 hours, PMN infiltration into the periodontal ligament from the orthodontic force probably results in the increased MPO level observed at this time point.
CONCLUSIONS: MPO might be a good marker to assess inflammation in orthodontic movement; it deserves further studies in orthodontic therapy.
Authors:
Andrea M Marcaccini; Patricia A F Amato; Fernanda V Leão; Raquel F Gerlach; Jose T L Ferreira
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics     Volume:  138     ISSN:  1097-6752     ISO Abbreviation:  Am J Orthod Dentofacial Orthop     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-08     Completed Date:  2010-12-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8610224     Medline TA:  Am J Orthod Dentofacial Orthop     Country:  United States    
Other Details:
Languages:  eng     Pagination:  613-6     Citation Subset:  D; IM    
Copyright Information:
Copyright © 2010 American Association of Orthodontists. Published by Mosby, Inc. All rights reserved.
Affiliation:
Department of Morphology, Stomatology and Physiology, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. amarcaccini@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Child
Female
Follow-Up Studies
Gingival Crevicular Fluid / enzymology*
Humans
Male
Neutrophil Infiltration / physiology
Neutrophils / enzymology
Orthodontic Wires*
Periodontal Ligament / pathology
Peroxidase / analysis*
Saliva / enzymology*
Stress, Mechanical
Tooth Movement / instrumentation
Chemical
Reg. No./Substance:
EC 1.11.1.7/Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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