Document Detail


Myeloid mineralocorticoid receptor controls macrophage polarization and cardiovascular hypertrophy and remodeling in mice.
MedLine Citation:
PMID:  20697155     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Inappropriate excess of the steroid hormone aldosterone, which is a mineralocorticoid receptor (MR) agonist, is associated with increased inflammation and risk of cardiovascular disease. MR antagonists are cardioprotective and antiinflammatory in vivo, and evidence suggests that they mediate these effects in part by aldosterone-independent mechanisms. Here we have shown that MR on myeloid cells is necessary for efficient classical macrophage activation by proinflammatory cytokines. Macrophages from mice lacking MR in myeloid cells (referred to herein as MyMRKO mice) exhibited a transcription profile of alternative activation. In vitro, MR deficiency synergized with inducers of alternatively activated macrophages (for example, IL-4 and agonists of PPARgamma and the glucocorticoid receptor) to enhance alternative activation. In vivo, MR deficiency in macrophages mimicked the effects of MR antagonists and protected against cardiac hypertrophy, fibrosis, and vascular damage caused by L-NAME/Ang II. Increased blood pressure and heart rates and decreased circadian variation were observed during treatment of MyMRKO mice with L-NAME/Ang II. We conclude that myeloid MR is an important control point in macrophage polarization and that the function of MR on myeloid cells likely represents a conserved ancestral MR function that is integrated in a transcriptional network with PPARgamma and glucocorticoid receptor. Furthermore, myeloid MR is critical for blood pressure control and for hypertrophic and fibrotic responses in the mouse heart and aorta.
Authors:
Michael G Usher; Sheng Zhong Duan; Christine Y Ivaschenko; Ryan A Frieler; Stefan Berger; Günther Schütz; Carey N Lumeng; Richard M Mortensen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-08-09
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  120     ISSN:  1558-8238     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-02     Completed Date:  2010-12-15     Revised Date:  2012-02-07    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3350-64     Citation Subset:  AIM; IM    
Affiliation:
Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, Michigan 48109-5622, USA.
Data Bank Information
Bank Name/Acc. No.:
GEO/GSE23308
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MeSH Terms
Descriptor/Qualifier:
Aldosterone / blood,  pharmacology
Animals
Blood Pressure / drug effects,  physiology
Cardiomegaly / pathology
Cardiovascular Diseases / pathology
Fibrosis / pathology
Heart / drug effects,  physiopathology
Hypertension / pathology,  physiopathology
Hypertrophy / pathology
Interleukin-4 / pharmacology
Macrophages / drug effects
Mice
Mice, Knockout
Mice, Transgenic
NG-Nitroarginine Methyl Ester / pharmacology
Receptors, Glucocorticoid / antagonists & inhibitors,  physiology
Receptors, Mineralocorticoid / antagonists & inhibitors*,  genetics,  physiology*
Grant Support
ID/Acronym/Agency:
K08 DK078851-05/DK/NIDDK NIH HHS; R01HL083201/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/NR3C1 protein, human; 0/Receptors, Glucocorticoid; 0/Receptors, Mineralocorticoid; 207137-56-2/Interleukin-4; 50903-99-6/NG-Nitroarginine Methyl Ester; 52-39-1/Aldosterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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