| Myelin proteolipid protein is not esterified at the site of synthesis. | |
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MedLine Citation:
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PMID: 20488003 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Brain slices prepared from 20-day old rats were incubated with [(3)H]palmitic acid to study its incorporation into myelin proteins. After separation by SDS-PAGE, most of the label was found to be associated with the major proteolipid protein (PLP) and with the intermediate protein (I). The radioactivity measured in PLP at short incubation times was shown to be due to palmitic acid bound to the protein by ester linkages. Time-course incorporation of [(3)H]palmitic acid into PLP of fraction SN(4) (a myelin like membrane) and of purified myelin showed that the former was poorly labeled and no relationship of the type 'precursor-product' between these fractions could be detected. Incorporation of the fatty acid into PLP was not affected by inhibition of the synthesis or transport of myelin PLP with cycloheximide or colchicine, indicating that the pool of PLP that can be acylated must be larger than the extramyelin pool. Addition of unlabeled palmitic acid to the incubation medium, 30 min after the addition of [(3)H]palmitate, stopped the appearance of label in myelin PLP almost immediately, indicating that there is no significant extramyelin pool of PLP destined for transport into myelin. The results presented in this paper strongly suggest that esterification of PLP takes place in the myelin membrane or at a site very close to it. |
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Authors:
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O A Bizzozero; E F Soto; J M Pasquini |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Neurochemistry international Volume: 5 ISSN: 0197-0186 ISO Abbreviation: Neurochem. Int. Publication Date: 1983 |
Date Detail:
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Created Date: 2010-05-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8006959 Medline TA: Neurochem Int Country: England |
Other Details:
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Languages: eng Pagination: 729-36 Citation Subset: - |
Affiliation:
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Departamento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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