Document Detail

Myelin proteolipid protein is not esterified at the site of synthesis.
MedLine Citation:
PMID:  20488003     Owner:  NLM     Status:  In-Data-Review    
Brain slices prepared from 20-day old rats were incubated with [(3)H]palmitic acid to study its incorporation into myelin proteins. After separation by SDS-PAGE, most of the label was found to be associated with the major proteolipid protein (PLP) and with the intermediate protein (I). The radioactivity measured in PLP at short incubation times was shown to be due to palmitic acid bound to the protein by ester linkages. Time-course incorporation of [(3)H]palmitic acid into PLP of fraction SN(4) (a myelin like membrane) and of purified myelin showed that the former was poorly labeled and no relationship of the type 'precursor-product' between these fractions could be detected. Incorporation of the fatty acid into PLP was not affected by inhibition of the synthesis or transport of myelin PLP with cycloheximide or colchicine, indicating that the pool of PLP that can be acylated must be larger than the extramyelin pool. Addition of unlabeled palmitic acid to the incubation medium, 30 min after the addition of [(3)H]palmitate, stopped the appearance of label in myelin PLP almost immediately, indicating that there is no significant extramyelin pool of PLP destined for transport into myelin. The results presented in this paper strongly suggest that esterification of PLP takes place in the myelin membrane or at a site very close to it.
O A Bizzozero; E F Soto; J M Pasquini
Related Documents :
241463 - Optimal conditions for protein synthesis in incubated slices of rat brain.
2194573 - Myristic acid is incorporated into the two acylatable domains of the functional glycopr...
20447363 - New alternatives to cosmetics preservation.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neurochemistry international     Volume:  5     ISSN:  0197-0186     ISO Abbreviation:  Neurochem. Int.     Publication Date:  1983  
Date Detail:
Created Date:  2010-05-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8006959     Medline TA:  Neurochem Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  729-36     Citation Subset:  -    
Departamento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  5-HT metabolism in the intestinal wall of the rat-I. The mucosa.
Next Document:  RNA synthesis in different regions of rat brain at various time intervals after cerebellectomy.