Document Detail


Myelin phagocytosis and remyelination of macrophage-enriched central nervous system aggregate cultures.
MedLine Citation:
PMID:  11746450     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An increased level of myelin basic protein (MBP) degradation peptide 80-89, representative of myelin breakdown, is detected in myelinating foetal rat brain aggregate cultures supplemented with peritoneal macrophages at a time coinciding with the onset of myelination. During the period of myelination, the proportion of activated macrophages/microglia in the aggregates decreases, accompanied by a reduction in the content of MBP degradation products. During the recovery period following a demyelinating episode, the rate of MBP synthesis in antibody-treated standard aggregates was greater than in their medium controls. However, the rate of MBP accumulation was not as efficient in macrophage-enriched aggregates and was associated with persistently raised MBP peptide levels. Thus, as occurs in multiple sclerosis lesions, attempts at remyelination appear to be counterbalanced by macrophage-mediated demyelination, with the continued presence of degraded myelin rendering a local environment that is not fully conducive to remyelination.
Authors:
C A Copelman; L T Diemel; D Gveric; N A Gregson; M L Cuzner
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  66     ISSN:  0360-4012     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-12-17     Completed Date:  2002-01-24     Revised Date:  2011-08-23    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1173-8     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Wiley-Liss, Inc.
Affiliation:
Department of Neurochemistry, Institute of Neurology, University College London, 1 Wakefield Street, London, WC1N 1PJ, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Antibodies / pharmacology
Antigens, CD*
Antigens, CD147
Antigens, Neoplasm*
Antigens, Surface*
Avian Proteins*
Blood Proteins*
Brain / embryology*,  immunology,  metabolism
Cell Count
Cell Size / immunology
Cells, Cultured / drug effects,  immunology,  metabolism
Ectodysplasins
Fetus
Humans
Immunohistochemistry
Macrophages / drug effects,  immunology,  metabolism*
Membrane Glycoproteins / metabolism
Membrane Proteins / metabolism
Middle Aged
Multiple Sclerosis / immunology,  metabolism*,  physiopathology
Myelin Basic Proteins / immunology,  metabolism*
Myelin Sheath / immunology,  metabolism*,  pathology
Myelin-Associated Glycoprotein / antagonists & inhibitors,  immunology,  metabolism
Nerve Regeneration / immunology*
Peptide Fragments / immunology,  metabolism
Phagocytosis / immunology*
Rats
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antigens, CD; 0/Antigens, Neoplasm; 0/Antigens, Surface; 0/Avian Proteins; 0/BSG protein, human; 0/Blood Proteins; 0/Bsg protein, Gallus gallus; 0/Bsg protein, rat; 0/EDA protein, human; 0/Ectodysplasins; 0/Membrane Glycoproteins; 0/Membrane Proteins; 0/Myelin Basic Proteins; 0/Myelin-Associated Glycoprotein; 0/Peptide Fragments; 0/myelin oligodendrocyte glycoprotein; 136894-56-9/Antigens, CD147

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