Document Detail


Myelin and microsomes from rhesus monkey spinal cord: isolation and effects of trauma.
MedLine Citation:
PMID:  6107873     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dispersions of rhesus monkey spinal cord and brain were separated into large particle (crude mitochondrial plus nuclear) and small particle (crude microsomal) fractions; myelin was isolated from each of these preparative fractions. In brain preparations, almost all myelin was found in the large particle fraction; in contrast, almost half the myelin from spinal cord preparations was found in the small particle fraction. In addition, much larger amounts of partially degraded myelin were found in the fraction floating on 0.32 M sucrose and in the cytosol fraction of the spinal cord preparation in comparison to those of the brain preparations. These results suggest that rhesus monkey spinal cord myelin is more fragile than brain myelin; upon dispersion of spinal cord, more small myelin vesicles (isolated from the crude microsomal fraction) and more 0.32 M sucrose floating fraction (partially degraded myelin) are formed. After trauma of the spinal cord, the proportion of small vesicle myelin was increased at the expense of large vesicle myelin, lending further support to the hypothesis that spinal cord myelin is more fragile than brain myelin. Although the lipid compositions were similar, spinal cord myelin had a lower protein content and a lower 2',3'-cyclic nucleotide phosphodiesterase specific activity than did brain myelin. The lipid composition of microsomes from brain differed somewhat from that of spinal cord microsomes.
Authors:
A D Toews; J S King; D Yashon; L A Horrocks
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neurological research     Volume:  1     ISSN:  0161-6412     ISO Abbreviation:  Neurol. Res.     Publication Date:  1980  
Date Detail:
Created Date:  1981-01-29     Completed Date:  1981-01-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7905298     Medline TA:  Neurol Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  271-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
2',3'-Cyclic-Nucleotide Phosphodiesterases / analysis
Animals
Brain / ultrastructure*
Macaca mulatta
Microscopy, Electron
Microsomes / analysis,  ultrastructure*
Myelin Sheath / analysis,  ultrastructure*
Nerve Tissue Proteins / analysis
Spinal Cord / ultrastructure*
Spinal Cord Injuries / metabolism,  pathology*
Grant Support
ID/Acronym/Agency:
NS-08291/NS/NINDS NIH HHS; NS-10165/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Nerve Tissue Proteins; EC 3.1.4.-/2',3'-Cyclic-Nucleotide Phosphodiesterases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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