Document Detail

Myelin-associated proteins block the migration of olfactory ensheathing cells: an in vitro study using single-cell tracking and traction force microscopy.
MedLine Citation:
PMID:  22205212     Owner:  NLM     Status:  MEDLINE    
Newly generated olfactory receptor axons grow from the peripheral to the central nervous system aided by olfactory ensheathing cells (OECs). Thus, OEC transplantation has emerged as a promising therapy for spinal cord injuries and for other neural diseases. However, these cells do not present a uniform population, but instead a functionally heterogeneous population that exhibits a variety of responses including adhesion, repulsion, and crossover during cell-cell and cell-matrix interactions. Some studies report that the migratory properties of OECs are compromised by inhibitory molecules and potentiated by chemical gradients. Here, we demonstrated that rodent OECs express all the components of the Nogo receptor complex and that their migration is blocked by myelin. Next, we used cell tracking and traction force microscopy to analyze OEC migration and its mechanical properties over myelin. Our data relate the decrease of traction force of OEC with lower migratory capacity over myelin, which correlates with changes in the F-actin cytoskeleton and focal adhesion distribution. Lastly, OEC traction force and migratory capacity is enhanced after cell incubation with the Nogo receptor inhibitor NEP1-40.
Sara Nocentini; Diego Reginensi; Simón Garcia; Patricia Carulla; María Teresa Moreno-Flores; Francisco Wandosell; Xavier Trepat; Ana Bribian; José A del Río
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-12-29
Journal Detail:
Title:  Cellular and molecular life sciences : CMLS     Volume:  69     ISSN:  1420-9071     ISO Abbreviation:  Cell. Mol. Life Sci.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-27     Completed Date:  2012-07-18     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  9705402     Medline TA:  Cell Mol Life Sci     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  1689-703     Citation Subset:  IM    
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MeSH Terms
Cell Movement*
Cell Tracking
GPI-Linked Proteins / physiology
Myelin Proteins / metabolism,  physiology*
Myelin Sheath / metabolism
Olfactory Bulb / cytology*,  metabolism
Rats, Sprague-Dawley
Receptors, Cell Surface / physiology
Grant Support
242993//European Research Council
Reg. No./Substance:
0/GPI-Linked Proteins; 0/Myelin Proteins; 0/Receptors, Cell Surface; 0/Rtn4r protein, rat

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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