Document Detail


Critical role of dispensable genes in Mycoplasma agalactiae interaction with mammalian cells.
MedLine Citation:
PMID:  20123713     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mycoplasmas are minimal bacteria whose genomes barely exceed the smallest amount of information required to sustain autonomous life. Despite this apparent simplicity, several mycoplasmas are successful pathogens of humans and animals, in which they establish intimate interactions with epithelial cells at mucosal surfaces. To identify biological functions mediating mycoplasma interactions with mammalian cells, we produced a library of transposon knockout mutants in the ruminant pathogen Mycoplasma agalactiae and used this library to identify mutants displaying a growth-deficient pheonotype in cell culture. M. agalactiae mutants displaying a 3-fold reduction in CFU titers to nearly complete extinction in coculture with HeLa cells were identified. Mapping of transposon insertion sites revealed 18 genomic regions putatively involved in the interaction of M. agalactiae with HeLa cells. Several of these regions encode proteins with features of membrane lipoproteins and/or were involved in horizontal gene transfer with phylogenetically distant pathogenic mycoplasmas of ruminants. Two mutants with the most extreme phenotype carry a transposon in a genomic region designated the NIF locus which encodes homologues of SufS and SufU, two proteins presumably involved in [Fe-S] cluster biosynthesis in Gram-positive bacteria. Complementation studies confirmed the conditional essentiality of the NIF locus, which was found to be critical for proliferation in the presence of HeLa cells and several other mammalian cell lines but dispensable for axenic growth. While our results raised questions regarding essential functions in mycoplasmas, they also provide a means for studying the role of mycoplasmas as minimal pathogens.
Authors:
Eric Baranowski; Sébastien Guiral; Eveline Sagné; Agnès Skapski; Christine Citti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-01
Journal Detail:
Title:  Infection and immunity     Volume:  78     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-17     Completed Date:  2010-04-16     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1542-51     Citation Subset:  IM    
Affiliation:
INRA, UMR 1225, F-31076 Toulouse, France.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion*
Bacterial Proteins / genetics,  physiology*
Coculture Techniques
Colony Count, Microbial
DNA Transposable Elements
Epithelial Cells / microbiology
Gene Knockout Techniques
Genes, Essential
Genetic Complementation Test
HeLa Cells
Humans
Mutagenesis, Insertional
Mycoplasma agalactiae / genetics,  growth & development,  pathogenicity*
Virulence Factors / genetics,  physiology*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA Transposable Elements; 0/Virulence Factors
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