Document Detail


Mycological survey for potential aflatoxin and ochratoxin producers and their toxicological properties in harvested Brazilian black pepper.
MedLine Citation:
PMID:  14726275     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A mycological survey was carried out on 115 samples of whole dried black pepper seeds, from two main production regions of Brazil (Pará and Espírito Santo). A high incidence of contamination was verified in both regions when 99.1% of the samples showed filamentous fungi contamination. A total of 497 species of nine different genera were isolated (Aspergillus, Eurotium, Rhizopus, Penicillium, Curvularia, Cladosporium, Absidia, Emericella and Paecilomyces). The genus Aspergillus was the predominant (53.5%) followed by species from the Eurotium genus (24.5%). Eurotium chevalieri (16.4%) was the most predominant species followed by A. flavus (14.6%) present on 55 samples of black pepper (47.8%) analysed. Twenty-five samples (21.7%) were contaminated with aflatoxigenic strains of A. flavus and A. parasiticus. In relation to the types of aflatoxins produced by mycotoxigenic strains, it was observed that 25 strains (44.6%) of 56 isolated of A. flavus produced aflatoxins. From 12 samples, A. ochraceus species were isolated in low frequency (3.5%). Two strains of A. ochraceus from 16 isolated were producers of ochratoxin A. With respect to the aflatoxins and ochratoxin A natural contamination, none of the samples presented detectable levels of these mycotoxins using thin-layer chromatographic analysis.
Authors:
M J Gatti; M E Fraga; C Magnoli; A M Dalcero; C A da Rocha Rosa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Food additives and contaminants     Volume:  20     ISSN:  0265-203X     ISO Abbreviation:  Food Addit Contam     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2004-01-16     Completed Date:  2004-03-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8500474     Medline TA:  Food Addit Contam     Country:  England    
Other Details:
Languages:  eng     Pagination:  1120-6     Citation Subset:  IM    
Affiliation:
Departamento de Biologia, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil.
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MeSH Terms
Descriptor/Qualifier:
Aflatoxins / biosynthesis*
Black Pepper / chemistry,  microbiology*
Brazil
Chromatography, Thin Layer / methods
Culture Media
Food Contamination / analysis
Food Microbiology*
Fungi / classification,  isolation & purification*,  metabolism
Humans
Ochratoxins / biosynthesis*
Chemical
Reg. No./Substance:
0/Aflatoxins; 0/Culture Media; 0/Ochratoxins; 303-47-9/ochratoxin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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