Document Detail


Mycolic acids from Mycobacterium tuberculosis: purification by countercurrent distribution and T-cell stimulation.
MedLine Citation:
PMID:  11491525     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacterial cell wall lipids are recognized as immunostimulatory molecules which make an important component of vaccines against bacterial diseases. Even mycolic acids, forming the waxy outer layer of the bacilli which cause tuberculosis, have been shown to stimulate human CD4/8 double negative T-cells. The role of these cells in resistance to tuberculosis is currently still debated. In this work, a method is described to purify mycolic acids from bacterial crude extracts in a single step using countercurrent distribution. Mycolic acids obtained in this way approach 100% purity and stimulate both double negative and CD4 positive T-cells in peripheral blood leucocytes obtained from healthy human donors. Stimulation of CD4 cells by mycolic acid antigens has not been reported before, emphasizing the potential importance of mycolic acids in the context of the fight against tuberculosis.
Authors:
M A Goodrum; D G Siko; T Niehues; D Eichelbauer; J A Verschoor
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microbios     Volume:  106     ISSN:  0026-2633     ISO Abbreviation:  Microbios     Publication Date:  2001  
Date Detail:
Created Date:  2001-08-08     Completed Date:  2002-05-07     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0207257     Medline TA:  Microbios     Country:  England    
Other Details:
Languages:  eng     Pagination:  55-67     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Faculty of Agricultural and Biological Sciences, University of Pretoria, South Africa.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD4
Antigens, CD8
Cell Culture Techniques
Cell Wall / metabolism
Chromatography, High Pressure Liquid
Cord Factors / metabolism
Countercurrent Distribution
Glycolipids / metabolism
Humans
Mycobacterium tuberculosis / immunology,  metabolism*
Mycolic Acids / analysis*,  immunology
T-Lymphocytes / immunology*
Trehalose / metabolism
Chemical
Reg. No./Substance:
0/Antigens, CD4; 0/Antigens, CD8; 0/Cord Factors; 0/Glycolipids; 0/Mycolic Acids; 99-20-7/Trehalose

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