Document Detail


Mycobacterium leprae induces insulin-like growth factor and promotes survival of Schwann cells upon serum withdrawal.
MedLine Citation:
PMID:  19732058     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Peripheral nerve lesions are considered the most relevant symptoms of leprosy, a chronic infectious disease caused by Mycobacterium leprae. The strategies employed by M. leprae to infect and multiply inside Schwann cells (SCs), however, remain poorly understood. In this study, it is shown that treatment of SCs with M. leprae significantly decreased cell death induced by serum deprivation. Not displayed by Mycobacterium smegmatis or Mycobacterium bovis BCG, the M. leprae survival effect was both dose dependent and specific. The conditioned medium (CM) of M. leprae-treated cultures was seen to mimic the protective effect of the bacteria, suggesting that soluble factors secreted by SCs in response to M. leprae were involved in cell survival. Indeed, by quantitative RT-PCR and dot blot/ELISA, it was demonstrated that M. leprae induced the expression and secretion of the SC survival factor insulin-like growth factor-I. Finally, the involvement of this hormone in M. leprae-induced SC survival was confirmed in experiments with neutralizing antibodies. Taken together, the results of this study delineate an important strategy for the successful colonization of M. leprae in the nerve based on the survival maintenance of the host cell through induction of IGF-I production.
Authors:
Luciana Silva Rodrigues; Elisa da Silva Maeda; Maria Elisabete Costa Moreira; Antonio Jorge Tempone; L?via Silva Lobato; Victor T?lio Ribeiro-Resende; Lucineia Alves; Shaila Rossle; Ulisses Gazos Lopes; Maria Cristina Vidal Pessolani
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-02
Journal Detail:
Title:  Cellular microbiology     Volume:  12     ISSN:  1462-5822     ISO Abbreviation:  Cell. Microbiol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-11     Completed Date:  2010-03-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883691     Medline TA:  Cell Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  42-54     Citation Subset:  IM    
Affiliation:
Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects
Cell Survival / drug effects,  physiology
Cells, Cultured
Culture Media, Conditioned / pharmacology
Culture Media, Serum-Free / pharmacology*
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Humans
Immunochemistry
Insulin-Like Growth Factor I / metabolism,  physiology*
Membrane Potential, Mitochondrial
Mycobacterium leprae / physiology*
Reverse Transcriptase Polymerase Chain Reaction
Schwann Cells / cytology,  drug effects,  metabolism*,  microbiology*
Chemical
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Culture Media, Serum-Free; 67763-96-6/Insulin-Like Growth Factor I

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