| Mycobacteriophage Ms6 LysB specifically targets the outer membrane of Mycobacterium smegmatis. | |
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MedLine Citation:
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PMID: 20093291 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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LysB, a mycobacteriophage Ms6-encoded protein, was previously identified as a lipolytic enzyme able to hydrolyse the ester bond in lipase and esterase substrates. In the present work, we show that LysB can hydrolyse lipids containing mycolic acids from the outer membrane of the mycobacterial cell wall. LysB was shown to hydrolyse the mycolic acids from the mycolyl-arabinogalactan-peptidoglycan complex where the mycolates of the inner leaflet of the outer membrane are covalently attached to an arabinosyl head group. In addition, treatment of the extractable lipids from Mycobacterium smegmatis, Mycobacterium bovis BCG and Mycobacterium tuberculosis H37Ra with LysB showed that trehalose 6,6'-dimycolate (TDM), a trehalose diester of two mycolic acid molecules, was hydrolysed by the enzyme. We have also determined the structures of the mycolic acid molecules that form the M. smegmatis TDM. The identification of a phage-encoded enzyme that targets the outer membrane of the mycobacterial cell wall enhances our understanding of the mechanism of mycobacteriophage lysis. |
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Authors:
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Filipa Gil; Anna E Grzegorzewicz; Maria João Catalão; João Vital; Michael R McNeil; Madalena Pimentel |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-01-21 |
Journal Detail:
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Title: Microbiology (Reading, England) Volume: 156 ISSN: 1465-2080 ISO Abbreviation: Microbiology (Reading, Engl.) Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-03 Completed Date: 2010-08-06 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 9430468 Medline TA: Microbiology Country: England |
Other Details:
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Languages: eng Pagination: 1497-504 Citation Subset: IM |
Affiliation:
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Unidade dos Retrovírus e Infecções Associadas Faculty of Pharmacy, University of Lisbon, Centro de Patogénese Molecular, Portugal. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Wall
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chemistry,
metabolism* Esters / metabolism Galactans / metabolism Hydrolysis Membrane Lipids / metabolism Mycobacteriophages / enzymology* Mycobacterium bovis / metabolism Mycobacterium smegmatis / metabolism* Mycobacterium tuberculosis / metabolism Mycolic Acids / metabolism Polysaccharides, Bacterial / metabolism Substrate Specificity Trehalose / metabolism Viral Proteins / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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AI33706/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Esters; 0/Galactans; 0/Membrane Lipids; 0/Mycolic Acids; 0/Polysaccharides, Bacterial; 0/Viral Proteins; 9036-66-2/arabinogalactan; 99-20-7/Trehalose |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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