Document Detail

Mycobacterial ES-31 serine protease--a biomarker for mycobacterium tuberculosis--a preliminary report.
MedLine Citation:
PMID:  20349755     Owner:  NLM     Status:  MEDLINE    
There is a need for simple and reliable method to identify Mycobacterium tuberculosis from AFB smear positive cases. Utility of mycobacterial ES-31 serine protease as a marker to detect Mycobacterium tuberculosis bacilli was explored using Fluorescein isothiocyanate conjugated anti-ES-31 serine protease antibody. The presence of ES-31 serine protease in bacilli was indicated by green fluorescence on the cell surface. Green fluorescence was observed with M.tb.H37Ra bacilli and M.tb.H37Rv bacilli while no Fluorescence was observed with M. chelonae, Nocardia farcinicum as well as in E. coli showing the usefulness of ES-31 serine protease as a marker for identification of mycobacterium tubercle bacilli in cultures.
M Anindita; V Upadhye; D Thamke; D K Mendiratta; B C Harinath
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Indian journal of tuberculosis     Volume:  56     ISSN:  0019-5707     ISO Abbreviation:  Indian J Tuberc     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2010-03-30     Completed Date:  2010-04-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0373027     Medline TA:  Indian J Tuberc     Country:  India    
Other Details:
Languages:  eng     Pagination:  141-3     Citation Subset:  IM    
Jamnalal Bajaj Tropical Disease Research Centre, Sevagram, Wardha.
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MeSH Terms
Antibodies, Bacterial / isolation & purification*
Fluorescent Antibody Technique
Green Fluorescent Proteins / metabolism
Mycobacterium tuberculosis / enzymology,  immunology,  isolation & purification*
Serine Proteases / metabolism
Reg. No./Substance:
0/Antibodies, Bacterial; 147336-22-9/Green Fluorescent Proteins; EC 3.4.-/Serine Proteases

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