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My Life with LIF: A Personal Account of Developing Laser-Induced Fluorescence.
MedLine Citation:
PMID:  22149473     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Laser-induced fluorescence (LIF) is a spectroscopic technique that involves the excitation of a molecular target by a beam of laser radiation followed by the detection of the subsequent emission of radiation from the target. LIF detection has several advantages over absorption spectroscopy. First, LIF has excellent detection sensitivity because a signal is observed against a dark background. Second, the emitted radiation can be collected at various angles with respect to the incoming laser beam, making it possible to obtain two- and three-dimensional images because the fluorescence is emitted in all directions. Third, by dispersing the fluorescence, it is also possible to learn about the transitions from the state excited to the various lower levels of the target species. Finally, because of the delay between the excitation and detection events, it is also possible to learn about what processes the excited target undergoes in the intervening time. Expected final online publication date for the Annual Review of Analytical Chemistry Volume 5 is June 15, 2012. Please see http://www.annualreviews.org/catalog/pubdates.aspx for revised estimates.
Authors:
Richard N Zare
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-6
Journal Detail:
Title:  Annual review of analytical chemistry (Palo Alto, Calif.)     Volume:  -     ISSN:  1936-1335     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-12-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101508602     Medline TA:  Annu Rev Anal Chem (Palo Alto Calif)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Chemistry, Stanford University, Stanford, CA 94305-5080.
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