Document Detail

Mutations responsible for reduced susceptibility to 4-quinolones in clinical isolates of multi-resistant Salmonella typhi in India.
MedLine Citation:
PMID:  8737139     Owner:  NLM     Status:  MEDLINE    
Twelve isolates of Salmonella typhi isolated in Vellore, India had reduced susceptibility to 4-quinolones (MIC of ciprofloxacin 0.256 mg/L). One isolate was isolated in 1992 but the remaining 11 were isolated in 1994. The section of the gyrA gene from codons 24 to 185, which includes the "Quinolone Resistance Determining Region", was amplified by the polymerase chain reaction and, after separating the amplified strands, the DNA was sequenced directly. In the one isolate from 1992, no alterations were seen in this region of gyrA, compared with the ciprofloxacin-sensitive isolates and presumably decreased 4-quinolone susceptibility resulted from reduced permeability of the cell outer membrane or another mechanism. In nine isolates from 1994, a substitution of phenylalanine for serine at position 83 of GyrA correlated with the decrease in 4-quinolone susceptibility. In the remaining two isolates, the novel substitution of aspartate to tyrosine at position 87 was found; in one isolate this substitution was coupled with another substitution at position 83 but in the other it was not.
J C Brown; P M Shanahan; M V Jesudason; C J Thomson; S G Amyes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of antimicrobial chemotherapy     Volume:  37     ISSN:  0305-7453     ISO Abbreviation:  J. Antimicrob. Chemother.     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-12-30     Completed Date:  1996-12-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7513617     Medline TA:  J Antimicrob Chemother     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  891-900     Citation Subset:  IM    
Department of Medical Microbiology, Medical School, University of Edinburgh, UK.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Ciprofloxacin / pharmacology
DNA Gyrase
DNA Topoisomerases, Type II / drug effects,  genetics*
Drug Resistance, Microbial / genetics*
Microbial Sensitivity Tests
Molecular Sequence Data
Nalidixic Acid / pharmacology
Ofloxacin / pharmacology
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Quinolones / pharmacology*
Salmonella typhi / drug effects*
Reg. No./Substance:
0/Quinolones; 389-08-2/Nalidixic Acid; 82419-36-1/Ofloxacin; 85721-33-1/Ciprofloxacin; EC 5.99.1.-/DNA Gyrase; EC Topoisomerases, Type II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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