Document Detail


Mutations in the nervous system--specific HSN2 exon of WNK1 cause hereditary sensory neuropathy type II.
MedLine Citation:
PMID:  18521183     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hereditary sensory and autonomic neuropathy type II (HSANII) is an early-onset autosomal recessive disorder characterized by loss of perception to pain, touch, and heat due to a loss of peripheral sensory nerves. Mutations in hereditary sensory neuropathy type II (HSN2), a single-exon ORF originally identified in affected families in Quebec and Newfoundland, Canada, were found to cause HSANII. We report here that HSN2 is a nervous system-specific exon of the with-no-lysine(K)-1 (WNK1) gene. WNK1 mutations have previously been reported to cause pseudohypoaldosteronism type II but have not been studied in the nervous system. Given the high degree of conservation of WNK1 between mice and humans, we characterized the structure and expression patterns of this isoform in mice. Immunodetections indicated that this Wnk1/Hsn2 isoform was expressed in sensory components of the peripheral nervous system and CNS associated with relaying sensory and nociceptive signals, including satellite cells, Schwann cells, and sensory neurons. We also demonstrate that the novel protein product of Wnk1/Hsn2 was more abundant in sensory neurons than motor neurons. The characteristics of WNK1/HSN2 point to a possible role for this gene in the peripheral sensory perception deficits characterizing HSANII.
Authors:
Masoud Shekarabi; Nathalie Girard; Jean-Baptiste Rivière; Patrick Dion; Martin Houle; André Toulouse; Ronald G Lafrenière; Freya Vercauteren; Pascale Hince; Janet Laganiere; Daniel Rochefort; Laurence Faivre; Mark Samuels; Guy A Rouleau
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  118     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-03     Completed Date:  2008-09-25     Revised Date:  2011-10-26    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2496-505     Citation Subset:  AIM; IM    
Affiliation:
Centre of Excellence in Neuromics, University of Montreal, Centre Hospitalier de l'Université de Montréal, Montreal, Quebec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Alternative Splicing
Amino Acid Sequence
Animals
Axons / metabolism
Central Nervous System / metabolism
Charcot-Marie-Tooth Disease / genetics*,  metabolism,  pathology
Female
Ganglia, Spinal / cytology,  metabolism
Gene Expression
Heterozygote
Humans
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Mutation*
Nerve Tissue Proteins / genetics*,  metabolism
Neuroglia / metabolism
Neurons / metabolism
Peripheral Nervous System / metabolism
Protein Isoforms / genetics,  metabolism
Protein-Serine-Threonine Kinases / genetics*,  metabolism
Sequence Deletion
Sequence Homology, Amino Acid
Spinal Nerve Roots / metabolism
Chemical
Reg. No./Substance:
0/Nerve Tissue Proteins; 0/Protein Isoforms; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/WNK1 protein, human
Comments/Corrections

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