| Mutations in the Saccharomyces cerevisiae vacuolar fusion proteins Ccz1, Mon1 and Ypt7 cause defects in cell cycle progression in a num1Delta background. | |
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MedLine Citation:
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PMID: 19700218 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The proteins Ccz1 and Mon1 are known to function together with the Rab-GTPase Ypt7 in membrane fusion reactions at the yeast vacuole. In a genome-wide analysis they have also been found to interact genetically with the nuclear-migration protein Num1. In this study we analyze these synthetic effects and we show that the mutants ccz1Delta num1Delta, mon1Delta num1Delta and ypt7Delta num1Delta exhibit severe defects in cell cycle progression. A large fraction of the mutant cells enter a new cell division cycle without having completed mitotic exit, leading to the accumulation of multinuclear, anuclear and multibudded cells. The double deletion strains display also increased sensitivity to calcium ions. The cell-cycle defects are only weakly observed if deletions of other vacuolar protein sorting genes are combined with num1Delta or if other nuclear-migration genes are deleted together with CCZ1, whereas the calcium sensitivity is characteristic for a large subset of the tested double mutants. Further, the cell-cycle defects of the ccz1Delta num1Delta strain can be partially rescued by overproduction of either the calcium pump Pmc1 or the nuclear-migration factors Kar9 and Bim1. Together, these results indicate that deregulation of the cell cycle in these mutants results from two separate mechanisms, one of which is related to calcium homeostasis. |
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Authors:
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Marta Hoffman-Sommer; R??za Kucharczyk; Iga Piekarska; Ewa Kozlowska; Joanna Rytka |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-08-22 |
Journal Detail:
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Title: European journal of cell biology Volume: 88 ISSN: 1618-1298 ISO Abbreviation: Eur. J. Cell Biol. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-09-14 Completed Date: 2009-12-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7906240 Medline TA: Eur J Cell Biol Country: Germany |
Other Details:
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Languages: eng Pagination: 639-52 Citation Subset: IM |
Affiliation:
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Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland. martah@ibb.waw.pl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Calcium
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metabolism Cell Cycle / genetics Cytoskeletal Proteins / chemistry, genetics*, metabolism Guanine Nucleotide Exchange Factors / chemistry, genetics, metabolism Microscopy, Fluorescence Mutation Saccharomyces cerevisiae / cytology*, genetics*, metabolism Saccharomyces cerevisiae Proteins / chemistry, genetics*, metabolism Vacuoles / metabolism Vesicular Transport Proteins / chemistry, genetics, metabolism rab GTP-Binding Proteins / chemistry, genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Ccz1 protein, S cerevisiae; 0/Cytoskeletal Proteins; 0/Guanine Nucleotide Exchange Factors; 0/Mon1 protein, S cerevisiae; 0/NUM1 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Vesicular Transport Proteins; 7440-70-2/Calcium; EC 3.6.1.-/YPT7 protein, S cerevisiae; EC 3.6.1.-/rab GTP-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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