Document Detail


Mutations in RNA polymerase II enhance or suppress mutations in GAL4.
MedLine Citation:
PMID:  2495535     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The activation domains of eukaryotic DNA-binding transcription factors, such as GAL4, may regulate transcription by contacting RNA polymerase II. One potential site on RNA polymerase II for such interactions is the C-terminal tandemly repeated heptapeptide domain in the largest subunit (RPO21). We have changed the number of heptapeptide repeats in this yeast RPO21 C-terminal domain and have expressed these mutant RNA polymerase II polypeptides in yeast cells containing either wild-type or defective GAL4 proteins. Although the number of RPO21 heptapeptide repeats had no effect on the activity of wild-type GAL4, changing the length of the C-terminal domain modified the ability of mutant GAL4 proteins to activate transcription. Shorter or longer RPO21 C-terminal domains enhanced or partially suppressed, respectively, the effects of deletions in the transcriptional-activation domains of GAL4. The same RPO21 mutations also affected transcriptional activation by a GAL4-GCN4 chimera. These data suggest that the activation domains of DNA-binding transcription factors could interact, either directly or indirectly, with the heptapeptide repeats of RNA polymerase II.
Authors:
L A Allison; C J Ingles
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  86     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1989 Apr 
Date Detail:
Created Date:  1989-05-24     Completed Date:  1989-05-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2794-8     Citation Subset:  IM    
Affiliation:
Banting and Best Department of Medical Research, University of Toronto, ON, Canada.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
DNA Mutational Analysis
DNA-Binding Proteins / genetics*
RNA Polymerase II / genetics*,  immunology
Saccharomyces cerevisiae / genetics*
Structure-Activity Relationship
Suppression, Genetic*
Transcription Factors / genetics*
Transcription, Genetic*
beta-Galactosidase / genetics
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Transcription Factors; EC 2.7.7.-/RNA Polymerase II; EC 3.2.1.23/beta-Galactosidase
Comments/Corrections

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