Document Detail


Mutations in POL1 increase the mitotic instability of tandem inverted repeats in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  8514147     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tandem inverted repeats (TIRs or hairpins) of 30 and 80 base-pair unit lengths are unstable mitotically in yeast (Saccharomyces cerevisiae). TIR instability results from deletions that remove part or all of the presumed hairpin structure from the chromosome. At least one deletion endpoint is always at or near the base of the hairpin, and almost all of the repaired junctions occur within short direct sequence repeats of 4 to 9 base pairs. The frequency of this event, which we call "hairpin excision," is influenced by chromosomal position, length of the inverted repeats, and the distance separating the repeat units; increasing the distance between the inverted repeats as little as 25 base pairs increases their chromosomal stability. The frequency of excision is not affected by representative rad mutations, but is influenced by mutations in certain genes affecting DNA synthesis. In particular, mutations in POL1/CDC17, the gene that encodes the large subunit of DNA polymerase I, increase the frequency of hairpin deletions significantly, implicating this protein in the normal maintainance of genomic TIRs.
Authors:
B Ruskin; G R Fink
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genetics     Volume:  134     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  1993 May 
Date Detail:
Created Date:  1993-07-20     Completed Date:  1993-07-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  43-56     Citation Subset:  IM    
Affiliation:
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
DNA Polymerase I / genetics
DNA, Fungal / genetics
Genes, Fungal*
Mitosis / genetics*
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
Recombination, Genetic
Repetitive Sequences, Nucleic Acid*
Saccharomyces cerevisiae / cytology,  enzymology,  genetics*
Sequence Deletion
Grant Support
ID/Acronym/Agency:
5R37GM35010/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Fungal; EC 2.7.7.-/DNA Polymerase I
Comments/Corrections

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