Document Detail


Mutations in the Drosophila glycoprotein hormone receptor, rickets, eliminate neuropeptide-induced tanning and selectively block a stereotyped behavioral program.
MedLine Citation:
PMID:  12151362     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adult insects achieve their final form shortly after adult eclosion by the combined effects of specialized behaviors that generate increased blood pressure, which causes cuticular expansion, and hormones, which plasticize and then tan the cuticle. We examined the molecular mechanisms contributing to these processes in Drosophila by analyzing mutants for the rickets gene. These flies fail to initiate the behavioral and tanning processes that normally follow ecdysis. Sequencing of rickets mutants and STS mapping of deficiencies confirmed that rickets encodes the glycoprotein hormone receptor DLGR2. Although rickets mutants produce and release the insect-tanning hormone bursicon, they do not melanize when injected with extracts containing bursicon. In contrast, mutants do melanize in response to injection of an analog of cyclic AMP, the second messenger for bursicon. Hence, rickets appears to encode a component of the bursicon response pathway, probably the bursicon receptor itself. Mutants also have a behavioral deficit in that they fail to initiate the behavioral program for wing expansion. A set of decapitation experiments utilizing rickets mutants and flies that lack cells containing the neuropeptide eclosion hormone, reveals a multicomponent control to the activation of this behavioral program.
Authors:
James D Baker; James W Truman
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of experimental biology     Volume:  205     ISSN:  0022-0949     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-08-01     Completed Date:  2003-02-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2555-65     Citation Subset:  IM    
Affiliation:
Department of Zoology, University of Washington, Box 351800 Seattle, WA 91895, USA. jabaker@ms.cc.sunysb.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Drosophila / genetics*,  growth & development,  physiology*
Drosophila Proteins / genetics*,  metabolism
Genes, Insect
Insect Hormones / metabolism,  physiology
Invertebrate Hormones / metabolism,  pharmacology
Molting
Mutation*
Receptors, Cell Surface / genetics*,  metabolism
Receptors, G-Protein-Coupled*
Sequence Tagged Sites
Stereotyped Behavior / drug effects,  physiology
Wing / growth & development
Grant Support
ID/Acronym/Agency:
T32 HD07183/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Insect Hormones; 0/Invertebrate Hormones; 0/LGR2 protein, Drosophila; 0/Receptors, Cell Surface; 0/Receptors, G-Protein-Coupled; 0/ecdysis-triggering hormone, Drosophila; 9041-06-9/bursicon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Sulforaphane and its glutathione conjugate but not sulforaphane nitrile induce UDP-glucuronosyl tran...
Next Document:  Social regulation of gonadotropin-releasing hormone.