Document Detail


Mutational analysis of the transcriptional activation domains of v-Myb.
MedLine Citation:
PMID:  11896590     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A minimal transcription activation domain of the v-Myb oncoprotein was initially mapped to a central cluster of charged residues using GAL4-Myb fusion proteins. This region has been proposed to interact directly with the CBP co-activator in animal cells. Regions flanking this central domain of v-Myb are required for transcriptional activation by the native, unfused protein in both mammalian cells and in budding yeast. To identify the critical residues for transcriptional activation, we have now subjected the minimal activation domain and flanking regions including the heptad leucine repeat to random PCR-mediated mutagenesis. We found that the entire region examined can endure extensive substitutions without affecting transcriptional activation by v-Myb in budding yeast. The few mutations that did affect transcriptional activation altered acidic residues within the minimal activation domain or the heptad leucine repeat region, rather than leucine residues. Remarkably, there was a strong concordance between transcriptional activation in animal cells and in budding yeast, even though budding yeast have no known homologue of CBP or related co-activators. In contrast, there was not a strong correlation between transcriptional activation and oncogenic transformation.
Authors:
Duen-Mei Wang; Joseph S Lipsick
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Oncogene     Volume:  21     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-03-22     Completed Date:  2002-04-15     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  England    
Other Details:
Languages:  eng     Pagination:  1611-5     Citation Subset:  IM    
Affiliation:
Department of Pathology, Stanford University School of Medicine, Stanford, California, CA 94305-5324, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amino Acid Substitution
Animals
Cell Transformation, Viral
Cells, Cultured
Chick Embryo
DNA Mutational Analysis
Genes, Reporter
Molecular Sequence Data
Oncogene Proteins v-myb / chemistry*,  genetics*,  metabolism
Saccharomycetales / genetics
Trans-Activators / chemistry*,  genetics*,  metabolism
Transcriptional Activation*
Grant Support
ID/Acronym/Agency:
R01 CA43592/CA/NCI NIH HHS; T32 CA09151/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Oncogene Proteins v-myb; 0/Trans-Activators

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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