Document Detail


Mutational analysis of regulated exocytosis in Tetrahymena.
MedLine Citation:
PMID:  9394019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Genetic analysis of regulated exocytosis can be accomplished in ciliates, since mutants defective in stimulus-dependent secretion of dense-core vesicles can be identified. In Tetrahymena thermophila, secretion in wild-type cells can result in their encapsulation by the proteins released from vesicle cores. Cells with defects in secretion were isolated from mutagenized homozygous cells that were generated using a highly efficient method. Screening was based both on a visual assay for encapsulation, and on a novel panning step using differential centrifugation to take advantage of the selective mobility of mutants that fail to encapsulate upon stimulation. 18 mutants with defects in several ordered steps have been identified. Defects in a set of these could be localized to three stages: granule formation, transport to cell surface docking sites, and exocytosis itself. Mutants with defects in this last stage can be ordered into successive steps based on several criteria, including their responsiveness to multiple secretagogues and Ca2+ ionophores. The results of both somatic and genetic complementation on selected pairs also help to characterize the defective factors.
Authors:
S M Melia; E S Cole; A P Turkewitz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cell science     Volume:  111 ( Pt 1)     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-02-25     Completed Date:  1998-02-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  131-40     Citation Subset:  IM    
Affiliation:
Department of Molecular Genetics, University of Chicago, Chicago, IL 60637, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport / physiology
Cytoplasmic Granules / physiology
DNA Mutational Analysis
Exocytosis / physiology*
Fluorescent Antibody Technique, Indirect
Membrane Fusion / physiology
Mutation / physiology
Tetrahymena thermophila / cytology*,  genetics*
Grant Support
ID/Acronym/Agency:
GM50946/GM/NIGMS NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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