Document Detail

Mutational analysis of the PsbL protein of photosystem II in the cyanobacterium Synechocystis sp. PCC 6803.
MedLine Citation:
PMID:  8512617     Owner:  NLM     Status:  MEDLINE    
The psbL gene is a member of the psbEFLJ gene cluster in the cyanobacterium Synechocystis sp. PCC 6803 and higher plants. psbL, a 4.5 kDa protein encoded by this gene, is a component of the photosystem II complex. The amino acid sequence of this protein indicates that it has a single membrane-spanning alpha-helical domain. We have used a targeted mutagenesis technique to delete the coding region of the psbL gene in Synechocystis 6803. The resultant mutant strain T345 did not show any PSII-mediated oxygen evolution activity and, as a result, could not grow under photoautotrophic conditions. However, it had normal PSI activity. The chlorophyll to phycobilin ratio in the T345 cells was significantly lower than that in the wild type cells. Fluorescence emission spectra (77 K) of the mutant cells showed the absence of a 695 nm band that usually originates from the PSII complex. Binding assays with radioactive diuron demonstrated that the mutant cells did not have any herbicide binding activity. However, immunostaining experiments showed that both the D 1 (the herbicide binding protein) and the D2 proteins of the PSII reaction center were present at > 25% of their normal levels in the thylakoid membranes of the T345 mutant cells. Our data indicate that the PsbL protein is essential for the normal functioning of PSII.
P R Anbudurai; H B Pakrasi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Zeitschrift für Naturforschung. C, Journal of biosciences     Volume:  48     ISSN:  0939-5075     ISO Abbreviation:  Z. Naturforsch., C, J. Biosci.     Publication Date:    1993 Mar-Apr
Date Detail:
Created Date:  1993-07-16     Completed Date:  1993-07-16     Revised Date:  2009-11-04    
Medline Journal Info:
Nlm Unique ID:  8912155     Medline TA:  Z Naturforsch C     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  267-74     Citation Subset:  IM    
Department of Biology, Washington University, St. Louis, MO 63130-4899.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Chlamydomonas reinhardtii / metabolism
Cyanobacteria / genetics,  metabolism*
DNA Mutational Analysis*
Electron Transport
Gene Deletion
Genes, Bacterial
Light-Harvesting Protein Complexes
Molecular Sequence Data
Multigene Family
Mutagenesis, Site-Directed
Oryza sativa / metabolism
Photosynthetic Reaction Center Complex Proteins / genetics,  metabolism*
Photosystem II Protein Complex*
Plants, Toxic
Protozoan Proteins / genetics,  metabolism*
Sequence Homology, Amino Acid
Tobacco / metabolism
Grant Support
Reg. No./Substance:
0/Light-Harvesting Protein Complexes; 0/Oligodeoxyribonucleotides; 0/Photosynthetic Reaction Center Complex Proteins; 0/Photosystem II Protein Complex; 0/Protozoan Proteins; 147651-82-9/photosystem II, psbL subunit

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