| A Mutation in the poxA Gene of Salmonella enterica Serovar Typhimurium Alters Protein Production, Elevates Susceptibility to Environmental Challenges, and Decreases Swine Colonization. | |
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MedLine Citation:
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PMID: 21348575 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Abstract Control of foodborne Salmonella within the farm-retail continuum is a complex issue since over 2500 serovars of Salmonella exist, the host range of Salmonella spp. varies greatly, and Salmonella is environmentally ubiquitous. To identify Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) genes important for pathogen survival, our research group previously screened a signature-tagged mutagenesis bank in an ex vivo swine stomach content assay. A mutation in the poxA gene, a member of the gene family encoding class-II aminoacyl-tRNA synthetases, decreased survival of Salmonella Typhimurium in the ex vivo swine stomach content assay. In the current study, complementation with a plasmid-encoded poxA gene restored survival of the poxA mutant to the level of the parental, wild-type strain. In vivo analysis of the poxA mutant in the natural porcine host revealed significantly reduced fecal shedding of Salmonella, decreased colonization of the tonsils, and decreased detection of the mutant strain in the cecal contents of the pigs at 7 days postinoculation (p < 0.05). Body temperature (fever) of the pigs inoculated with wild-type Salmonella Typhimurium was significantly higher than that of pigs inoculated with the poxA mutant (p < 0.05). Two-dimensional gel electrophoresis revealed characteristic differences in the protein profile of the poxA mutant relative to the wild-type strain, indicating that deletion of poxA in Salmonella Typhimurium exerts selective effects on translation and/or posttranslational modifications of mRNA species that are necessary for stress survival and colonization of the natural swine host. |
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Authors:
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Shawn M D Bearson; Bradley L Bearson; Brian W Brunelle; Vijay K Sharma; In Soo Lee |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-2-24 |
Journal Detail:
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Title: Foodborne pathogens and disease Volume: - ISSN: 1556-7125 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-2-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101120121 Medline TA: Foodborne Pathog Dis Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1 Food Safety and Enteric Pathogens Research Unit, National Animal Disease Center , Ames, Iowa . |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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