Document Detail


Mutation in Rab11 results in abnormal organization of ommatidial cells and activation of JNK signaling in the Drosophila eye.
MedLine Citation:
PMID:  19473727     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rab11(mo), a P insertion line of Rab11 showed degenerated ommatidia and excess cell death in larval/pupal eyes. Here, we demonstrate that Rab11 is essential for normal organization of ommatidial cells and their survival in Drosophila, and a mutation in this gene results in cytoskeleton disruption and activation of JNK signaling in the eye. The spatial organization of various cell types in compound eye, viz., cone, photoreceptor, pigment and bristle cells, were disrupted in Rab11 mutants as revealed by immunostaining of F-actin and adherens and septate junction proteins. Genetic interaction studies indicated that mutation in Rab11 upregulates Drosophila apoptotic genes, rpr, hid and grim. In order to study the pathway that causes excessive cell death in Rab11 mutants, the JNK pathway was chosen and genetic interaction analyses were carried out between Rab11 and candidates of the JNK signaling pathway. A downregulation of JNK signaling rescued the phenotype in Rab11 mutant eyes significantly while overexpression of JNK in the eyes using UAS-eiger, UAS-dtak1 or EP(2)0578, resulted in enhancement of the eye phenotype indicating a link between Rab11 and the JNK signaling pathway.
Authors:
Anand K Tiwari; Jagat K Roy
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-26
Journal Detail:
Title:  European journal of cell biology     Volume:  88     ISSN:  1618-1298     ISO Abbreviation:  Eur. J. Cell Biol.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-06-22     Completed Date:  2009-09-09     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7906240     Medline TA:  Eur J Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  445-60     Citation Subset:  IM    
Affiliation:
Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221005, India.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis
Compound Eye, Arthropod* / metabolism,  pathology
Cytoskeleton / metabolism
Drosophila Proteins / biosynthesis,  genetics
Drosophila melanogaster
JNK Mitogen-Activated Protein Kinases / metabolism*
Membrane Proteins / biosynthesis,  genetics
Mutation*
Neuropeptides / biosynthesis,  genetics
Photoreceptor Cells, Invertebrate / metabolism,  pathology*
Signal Transduction / genetics
rab GTP-Binding Proteins / physiology*
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Membrane Proteins; 0/Neuropeptides; 0/eiger protein, Drosophila; 0/grim protein, Drosophila; 0/reaper protein, Drosophila; 0/wrinkled protein, Drosophila; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases; EC 3.6.1.-/rab GTP-Binding Proteins; EC 3.6.1.-/rab11 protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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