Document Detail


Mutation in GDP-fucose synthesis genes of Sinorhizobium fredii alters Nod factors and significantly decreases competitiveness to nodulate soybeans.
MedLine Citation:
PMID:  10065558     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We mutagenized Sinorhizobium fredii HH103-1 with Tn5-B20 and screened about 2,000 colonies for increased beta-galactosidase activity in the presence of the flavonoid naringenin. One mutant, designated SVQ287, produces lipochitooligosaccharide Nod factors (LCOs) that differ from those of the parental strain. The nonreducing N-acetylglucosamine residues of all of the LCOs of mutant SVQ287 lack fucose and 2-O-methylfucose substituents. In addition, SVQ287 synthesizes an LCO with an unusually long, C20:1 fatty acyl side chain. The transposon insertion of mutant SVQ287 lies within a 1.1-kb HindIII fragment. This and an adjacent 2.4-kb HindIII fragment were sequenced. The sequence contains the 3' end of noeK, nodZ, and noeL (the gene interrupted by Tn5-B20), and the 5' end of nolK, all in the same orientation. Although each of these genes has a similarly oriented counterpart on the symbiosis plasmid of the broad-host-range Rhizobium sp. strain NGR234, there are significant differences in the noeK/nodZ intergenic region. Based on amino acid sequence homology, noeL encodes GDP-D-mannose dehydratase, an enzyme involved in the synthesis of GDP-L-fucose, and nolK encodes a NAD-dependent nucleotide sugar epimerase/dehydrogenase. We show that expression of the noeL gene is under the control of NodD1 in S. fredii and is most probably mediated by the nod box that precedes nodZ. Transposon insertion into neoL has two impacts on symbiosis with Williams soybean: nodulation rate is reduced slightly and competitiveness for nodulation is decreased significantly. Mutant SVQ287 retains its ability to form nitrogen-fixing nodules on other legumes, but final nodule number is attenuated on Cajanus cajan.
Authors:
Y Lamrabet; R A Bellogín; T Cubo; R Espuny; A Gil; H B Krishnan; M Megias; F J Ollero; S G Pueppke; J E Ruiz-Sainz; H P Spaink; P Tejero-Mateo; J Thomas-Oates; J M Vinardell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular plant-microbe interactions : MPMI     Volume:  12     ISSN:  0894-0282     ISO Abbreviation:  Mol. Plant Microbe Interact.     Publication Date:  1999 Mar 
Date Detail:
Created Date:  1999-03-22     Completed Date:  1999-03-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9107902     Medline TA:  Mol Plant Microbe Interact     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  207-17     Citation Subset:  IM    
Affiliation:
Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, Spain.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF072888
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Carbohydrate Sequence
DNA, Bacterial
Guanosine Diphosphate Fucose / biosynthesis*
Membrane Proteins*
Molecular Sequence Data
Mutation*
Nitrogen Fixation / genetics*
Open Reading Frames
Phenotype
Plant Proteins / genetics*
Rhizobium / enzymology,  genetics*
Sequence Homology, Amino Acid
Soybeans / physiology*
beta-Galactosidase / metabolism
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/Membrane Proteins; 0/Plant Proteins; 0/nodulin; 15839-70-0/Guanosine Diphosphate Fucose; EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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