Document Detail

Mutation of a UDP-glucose-4-epimerase alters nematode susceptibility and ethylene responses in Arabidopsis roots.
MedLine Citation:
PMID:  15546354     Owner:  NLM     Status:  MEDLINE    
In Arabidopsis, mutation of RHD1, a UDP-glucose-4-epimerase, causes root-specific phenotypes, including hypersusceptibility to the cyst nematode Heterodera schachtii, increased root hair elongation, decreased root length, and root epidermal bulging. Previous experiments suggested that increased ethylene sensitivity or production mediated the rhd1-4 phenotypes. In the present study, double mutant analyses revealed that only rhd1-4 hypersusceptibility to H. schachtii and increased root hair elongation were dependent upon the ethylene signaling genes EIN2 and EIN3 but not upon ethylene signaling mediated by the auxin efflux carrier EIR1. In contrast, the rhd1-4 short root and root epidermal bulging phenotypes did not require EIN2, EIN3, or EIR1. A time-course analysis of RHD1 transcript levels in wild-type plants treated with the ethylene precursor 1-aminocyclopropane-1-carboxylic acid showed a root-specific downregulation of RHD1 expression by ethylene. This observation was corroborated by our finding of increased RHD1 transcript levels in roots of the ethylene-insensitive mutants etr1 and ein2. In addition to ethylene, auxin strongly influences H. schachtii susceptibility and root hair elongation. Therefore, we investigated the sensitivity of rhd1-4 roots to indole-3-acetic acid (IAA). Equivalent IAA concentrations caused a greater reduction in rhd1-4 root elongation compared with wild-type roots. Finally, H. schachtii parasitism was found to strongly downregulate RHD1 expression in the root 3 days after inoculation. We conclude that RHD1 is a likely target of root-specific negative regulation by ethylene and that loss of RHD1 function results in a heightened sensitivity of root tissues to both ethylene and auxin.
Martin J E Wubben; Steven R Rodermel; Thomas J Baum
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  40     ISSN:  0960-7412     ISO Abbreviation:  Plant J.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-19     Completed Date:  2005-01-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  712-24     Citation Subset:  IM    
Interdepartmental Genetics Program, Iowa State University, Ames, IA 50011, USA.
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MeSH Terms
Amino Acids, Cyclic / metabolism,  pharmacology
Arabidopsis / drug effects,  parasitology,  physiology*
Arabidopsis Proteins / genetics,  metabolism*
Culture Media
Ethylenes / metabolism*
Gene Expression Regulation, Plant
Indoleacetic Acids / pharmacology
Nematoda / physiology*
Plant Diseases / genetics
Plant Growth Regulators / physiology
Plant Roots / growth & development,  metabolism
Signal Transduction
UDPglucose 4-Epimerase / genetics,  metabolism*
Reg. No./Substance:
0/Amino Acids, Cyclic; 0/Arabidopsis Proteins; 0/Culture Media; 0/Ethylenes; 0/Indoleacetic Acids; 0/Plant Growth Regulators; 22059-21-8/1-aminocyclopropane-1-carboxylic acid; 74-85-1/ethylene; 87-51-4/indoleacetic acid; EC protein, Arabidopsis; EC 4-Epimerase

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