Document Detail


Zfp423/OAZ mutation reveals the importance of Olf/EBF transcription activity in olfactory neuronal maturation.
MedLine Citation:
PMID:  23035080     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Zfp423/OAZ, a multi-zinc finger protein, is proposed to participate in neuronal differentiation through interactions with the Olf/EBF (O/E) family of transcription factors and mediate extrinsic BMP signaling pathways. These activities are associated with distinct domains of the Olf/EBF-associated zinc finger (OAZ) protein. Sustained OAZ expression arrests olfactory sensory neurons (OSNs) at an immature state and alters olfactory receptor expression, but the mechanism remains elusive. We show here that constitutive expression of a C-terminal mutant OAZ (OAZΔC) in mice that selectively disrupts OAZ-O/E interaction while retaining other activities, exhibits apparently normal OSN differentiation. Additionally, interfering with potential BMP signaling pathways by inducible Follistatin expression in adult mice does not alter the neuronal lineage or differentiation status. Our results indicate that O/E-mediated processes are essential for the differentiation of OSNs and the establishment of a mature phenotype. BMP signaling pathways, if they are active in normal adult olfactory epithelium, may play a minor role in this tissue.
Authors:
Yang A Roby; Michael A Bushey; Li E Cheng; Heather M Kulaga; Se-Jin Lee; Randall R Reed
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  32     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-04     Completed Date:  2013-01-17     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  13679-88a     Citation Subset:  IM    
Affiliation:
Center for Sensory Biology, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Basic Helix-Loop-Helix Transcription Factors / physiology*
Bone Morphogenetic Proteins / physiology
Cell Lineage
DNA-Binding Proteins / chemistry,  genetics*,  physiology
Follistatin / biosynthesis,  genetics,  physiology
Gene Expression Regulation, Developmental
Genes, Reporter
Helix-Loop-Helix Motifs
Mice
Mice, Inbred C57BL
Neurogenesis / genetics*
Olfactory Mucosa / cytology
Olfactory Receptor Neurons / cytology*,  metabolism
Phenotype
Point Mutation*
Protein Binding
Protein Interaction Mapping
Protein Structure, Tertiary
Receptors, Odorant / genetics,  physiology*
Recombinant Fusion Proteins / physiology
Signal Transduction / physiology
Structure-Activity Relationship
Transcription Factors / chemistry,  genetics*,  physiology
Transcription, Genetic*
Zinc Fingers / genetics*,  physiology
Grant Support
ID/Acronym/Agency:
DC008295/DC/NIDCD NIH HHS; HD35887/AR060636/AR/NIAMS NIH HHS; R01 AR060636/AR/NIAMS NIH HHS; R01 DC004553/DC/NIDCD NIH HHS; R01 DC008295/DC/NIDCD NIH HHS
Chemical
Reg. No./Substance:
0/Basic Helix-Loop-Helix Transcription Factors; 0/Bone Morphogenetic Proteins; 0/DNA-Binding Proteins; 0/Ebf2 protein, mouse; 0/Ebfaz protein, mouse; 0/FST protein, human; 0/Follistatin; 0/Receptors, Odorant; 0/Recombinant Fusion Proteins; 0/Transcription Factors
Comments/Corrections

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