| Zfp423/OAZ mutation reveals the importance of Olf/EBF transcription activity in olfactory neuronal maturation. | |
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MedLine Citation:
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PMID: 23035080 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Zfp423/OAZ, a multi-zinc finger protein, is proposed to participate in neuronal differentiation through interactions with the Olf/EBF (O/E) family of transcription factors and mediate extrinsic BMP signaling pathways. These activities are associated with distinct domains of the Olf/EBF-associated zinc finger (OAZ) protein. Sustained OAZ expression arrests olfactory sensory neurons (OSNs) at an immature state and alters olfactory receptor expression, but the mechanism remains elusive. We show here that constitutive expression of a C-terminal mutant OAZ (OAZΔC) in mice that selectively disrupts OAZ-O/E interaction while retaining other activities, exhibits apparently normal OSN differentiation. Additionally, interfering with potential BMP signaling pathways by inducible Follistatin expression in adult mice does not alter the neuronal lineage or differentiation status. Our results indicate that O/E-mediated processes are essential for the differentiation of OSNs and the establishment of a mature phenotype. BMP signaling pathways, if they are active in normal adult olfactory epithelium, may play a minor role in this tissue. |
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Authors:
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Yang A Roby; Michael A Bushey; Li E Cheng; Heather M Kulaga; Se-Jin Lee; Randall R Reed |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: The Journal of neuroscience : the official journal of the Society for Neuroscience Volume: 32 ISSN: 1529-2401 ISO Abbreviation: J. Neurosci. Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-04 Completed Date: 2013-01-17 Revised Date: 2013-04-05 |
Medline Journal Info:
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Nlm Unique ID: 8102140 Medline TA: J Neurosci Country: United States |
Other Details:
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Languages: eng Pagination: 13679-88a Citation Subset: IM |
Affiliation:
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Center for Sensory Biology, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Basic Helix-Loop-Helix Transcription Factors / physiology* Bone Morphogenetic Proteins / physiology Cell Lineage DNA-Binding Proteins / chemistry, genetics*, physiology Follistatin / biosynthesis, genetics, physiology Gene Expression Regulation, Developmental Genes, Reporter Helix-Loop-Helix Motifs Mice Mice, Inbred C57BL Neurogenesis / genetics* Olfactory Mucosa / cytology Olfactory Receptor Neurons / cytology*, metabolism Phenotype Point Mutation* Protein Binding Protein Interaction Mapping Protein Structure, Tertiary Receptors, Odorant / genetics, physiology* Recombinant Fusion Proteins / physiology Signal Transduction / physiology Structure-Activity Relationship Transcription Factors / chemistry, genetics*, physiology Transcription, Genetic* Zinc Fingers / genetics*, physiology |
| Grant Support | |
ID/Acronym/Agency:
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DC008295/DC/NIDCD NIH HHS; HD35887/AR060636/AR/NIAMS NIH HHS; R01 DC004553/DC/NIDCD NIH HHS; R01 DC008295/DC/NIDCD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Basic Helix-Loop-Helix Transcription Factors; 0/Bone Morphogenetic Proteins; 0/DNA-Binding Proteins; 0/Ebf2 protein, mouse; 0/Ebfaz protein, mouse; 0/FST protein, human; 0/Follistatin; 0/Receptors, Odorant; 0/Recombinant Fusion Proteins; 0/Transcription Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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