Document Detail


Mutation of Drosophila Lsd1 disrupts H3-K4 methylation, resulting in tissue-specific defects during development.
MedLine Citation:
PMID:  17462898     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Histone-tail modifications play a fundamental role in the processes that establish chromatin structure and determine gene expression. One such modification, histone methylation, was considered irreversible until the recent discovery of histone demethylases. Lsd1 was the first histone demethylase to be identified. Lsd1 is highly conserved in many species, from yeast to humans, but its function has primarily been studied through biochemical approaches. The mammalian ortholog has been shown to demethylate monomethyl- and dimethyl-K4 and -K9 residues of histone H3. Here we describe the effects of Lsd1 mutation in Drosophila. The inactivation of dLsd1 strongly affects the global level of monomethyl- and dimethyl-H3-K4 methylation and results in elevated expression of a subset of genes. dLsd1 is not an essential gene, but animal viability is strongly reduced in mutant animals in a gender-specific manner. Interestingly, dLsd1 mutants are sterile and possess defects in ovary development, indicating that dLsd1 has tissue-specific functions. Mutant alleles of dLsd1 suppress positional-effect variegation, suggesting a disruption of the balance between euchromatin and heterochromatin. Taken together, these results show that dLsd1-mediated H3-K4 demethylation has a significant and specific role in Drosophila development.
Authors:
Luisa Di Stefano; Jun-Yuan Ji; Nam-Sung Moon; Anabel Herr; Nicholas Dyson
Related Documents :
20595228 - New chaps in the histone chaperone arena.
16630818 - Control of developmental regulators by polycomb in human embryonic stem cells.
19703438 - Histone h3 lysine 4 (h3k4) methylation in development and differentiation.
18201968 - Stat3 and oct-3/4 control histone modification through induction of eed in embryonic st...
6090458 - Identification of a camp regulatory region in the gene for rat cytosolic phosphoenolpyr...
12589088 - Tissue plasminogen activator and plasminogen activator inhibitor type 1 gene polymorphi...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Current biology : CB     Volume:  17     ISSN:  0960-9822     ISO Abbreviation:  Curr. Biol.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-01     Completed Date:  2007-12-12     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  9107782     Medline TA:  Curr Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  808-12     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
DNA Methylation*
Drosophila / embryology,  genetics*
Drosophila Proteins / genetics,  metabolism*
Gene Expression Regulation, Developmental*
Histones / metabolism*
Mutation / genetics
Oxidoreductases, N-Demethylating / genetics,  metabolism*
Phenotype*
Grant Support
ID/Acronym/Agency:
CA64402/CA/NCI NIH HHS; P01 CA095281/CA/NCI NIH HHS; P01 CA095281-01A10001/CA/NCI NIH HHS; R01 CA064402/CA/NCI NIH HHS; R01 CA064402-12/CA/NCI NIH HHS; R01 CA064402-13/CA/NCI NIH HHS; R01 GM053203/GM/NIGMS NIH HHS; R01 GM053203-11/GM/NIGMS NIH HHS; R01 GM053203-12/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Histones; EC 1.5.-/Lsd1 protein, Drosophila; EC 1.5.-/Oxidoreductases, N-Demethylating
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Chemotaxis in the absence of PIP3 gradients.
Next Document:  Joint stiffness of the ankle during walking after successful mobile-bearing total ankle replacement.