Document Detail


Mutagenicity of methylisocyanate and its reaction products to cultured mammalian cells.
MedLine Citation:
PMID:  3736578     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Methylisocyanate (MIC) induced mutagenic responses in the absence of exogenous activation in the mouse lymphoma cell forward mutation assay at concentrations as low as 8-24 microM. MIC produced predominantly small mutant colonies, suggesting the possibility of clastogenic activity. The intermediate hydrolysis product, methylamine, was also mutagenic without exogenous activation but required several hundred-fold higher concentrations (ca. 3 mM). N,N'-Dimethylurea, the final product in the reaction of methylisocyanate and water, was totally refractory in either the presence or absence of S9 for concentrations up to 57 mM (5 mg/ml). The ethyl ester of N-methylcarbamic acid was also tested since it was the only available analogue to the highly reactive N-methylcarbamic acid intermediate. This compound was mutagenic only in the presence of S9 at doses exceeding 5-40 microM, which suggested the possibility that the free acid, produced by enzymatic hydrolysis, is also mutagenic. The mutagenic activity of the ester resulted solely in the production of small mutant colonies.
Authors:
W J Caspary; B Myhr
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Mutation research     Volume:  174     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1986 Aug 
Date Detail:
Created Date:  1986-09-17     Completed Date:  1986-09-17     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  285-93     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biotransformation
Cells, Cultured
Cyanates / pharmacology*
Isocyanates*
Leukemia L5178
Methylurea Compounds / pharmacology
Mice
Microsomes, Liver / metabolism
Mutagenicity Tests
Rats
Urethane / analogs & derivatives,  pharmacology
Chemical
Reg. No./Substance:
0/Cyanates; 0/Isocyanates; 0/Methylurea Compounds; 105-40-8/N-methylcarbamic acid, ethyl ester; 51-79-6/Urethane; 598-94-7/1,1-dimethylurea; 624-83-9/methyl isocyanate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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