Document Detail

Mutagenicity of methylisocyanate and its reaction products to cultured mammalian cells.
MedLine Citation:
PMID:  3736578     Owner:  NLM     Status:  MEDLINE    
Methylisocyanate (MIC) induced mutagenic responses in the absence of exogenous activation in the mouse lymphoma cell forward mutation assay at concentrations as low as 8-24 microM. MIC produced predominantly small mutant colonies, suggesting the possibility of clastogenic activity. The intermediate hydrolysis product, methylamine, was also mutagenic without exogenous activation but required several hundred-fold higher concentrations (ca. 3 mM). N,N'-Dimethylurea, the final product in the reaction of methylisocyanate and water, was totally refractory in either the presence or absence of S9 for concentrations up to 57 mM (5 mg/ml). The ethyl ester of N-methylcarbamic acid was also tested since it was the only available analogue to the highly reactive N-methylcarbamic acid intermediate. This compound was mutagenic only in the presence of S9 at doses exceeding 5-40 microM, which suggested the possibility that the free acid, produced by enzymatic hydrolysis, is also mutagenic. The mutagenic activity of the ester resulted solely in the production of small mutant colonies.
W J Caspary; B Myhr
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Mutation research     Volume:  174     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1986 Aug 
Date Detail:
Created Date:  1986-09-17     Completed Date:  1986-09-17     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  285-93     Citation Subset:  IM    
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MeSH Terms
Cells, Cultured
Cyanates / pharmacology*
Leukemia L5178
Methylurea Compounds / pharmacology
Microsomes, Liver / metabolism
Mutagenicity Tests
Urethane / analogs & derivatives,  pharmacology
Reg. No./Substance:
0/Cyanates; 0/Isocyanates; 0/Methylurea Compounds; 105-40-8/N-methylcarbamic acid, ethyl ester; 51-79-6/Urethane; 598-94-7/1,1-dimethylurea; 624-83-9/methyl isocyanate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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