| Mutagenicity of methylisocyanate and its reaction products to cultured mammalian cells. | |
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MedLine Citation:
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PMID: 3736578 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Methylisocyanate (MIC) induced mutagenic responses in the absence of exogenous activation in the mouse lymphoma cell forward mutation assay at concentrations as low as 8-24 microM. MIC produced predominantly small mutant colonies, suggesting the possibility of clastogenic activity. The intermediate hydrolysis product, methylamine, was also mutagenic without exogenous activation but required several hundred-fold higher concentrations (ca. 3 mM). N,N'-Dimethylurea, the final product in the reaction of methylisocyanate and water, was totally refractory in either the presence or absence of S9 for concentrations up to 57 mM (5 mg/ml). The ethyl ester of N-methylcarbamic acid was also tested since it was the only available analogue to the highly reactive N-methylcarbamic acid intermediate. This compound was mutagenic only in the presence of S9 at doses exceeding 5-40 microM, which suggested the possibility that the free acid, produced by enzymatic hydrolysis, is also mutagenic. The mutagenic activity of the ester resulted solely in the production of small mutant colonies. |
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Authors:
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W J Caspary; B Myhr |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Mutation research Volume: 174 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 1986 Aug |
Date Detail:
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Created Date: 1986-09-17 Completed Date: 1986-09-17 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 285-93 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biotransformation Cells, Cultured Cyanates / pharmacology* Isocyanates* Leukemia L5178 Methylurea Compounds / pharmacology Mice Microsomes, Liver / metabolism Mutagenicity Tests Rats Urethane / analogs & derivatives, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Cyanates; 0/Isocyanates; 0/Methylurea Compounds; 105-40-8/N-methylcarbamic acid, ethyl ester; 51-79-6/Urethane; 598-94-7/1,1-dimethylurea; 624-83-9/methyl isocyanate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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