Document Detail

Mutagenic effects of 8-hydroxy-dGTP in live mammalian cells.
MedLine Citation:
PMID:  17448902     Owner:  NLM     Status:  MEDLINE    
The mutagenicity of an oxidized form of dGTP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP), was examined using COS-7 cells. 8-OH-dGTP and supF shuttle plasmid DNA were cointroduced by means of cationic liposomes, and the DNAs replicated in the cells were recovered and then transfected into Escherichia coli. 8-OH-dGTP induced A:T-->C:G substitution mutations in the COS-7 cells. This result agrees with previous observations indicating that DNA polymerases misincorporate 8-OH-dGTP opposite A in vitro, and that the oxidized deoxyribonucleotide induces A:T-->C:G transversions in E. coli. These results constitute the first direct evidence to show that 8-OH-dGTP actually induces mutations in living mammalian cells.
Kazuya Satou; Kazuaki Kawai; Hiroshi Kasai; Hideyoshi Harashima; Hiroyuki Kamiya
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-28
Journal Detail:
Title:  Free radical biology & medicine     Volume:  42     ISSN:  0891-5849     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-23     Completed Date:  2007-07-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1552-60     Citation Subset:  IM    
Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Sapporo 060-0812, Japan.
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MeSH Terms
COS Cells
Cations / chemistry
Cercopithecus aethiops
DNA / drug effects*,  genetics
DNA Replication
DNA-Directed DNA Polymerase / metabolism
Deoxyguanine Nucleotides / toxicity*
Escherichia coli Proteins / genetics
Liposomes / chemistry
Plasmids / chemistry,  drug effects,  genetics
Reg. No./Substance:
0/8-hydroxy-dGTP; 0/Cations; 0/Deoxyguanine Nucleotides; 0/Escherichia coli Proteins; 0/Liposomes; 0/Suf E protein, E coli; 9007-49-2/DNA; EC DNA Polymerase

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