Document Detail

Mutagenic activation of environmental carcinogens by microsomes of gastric mucosa with intestinal metaplasia.
MedLine Citation:
PMID:  10463577     Owner:  NLM     Status:  MEDLINE    
Coexpression of cytochrome P450 monooxygenases (CYPs) and reductase was found in human gastric mucosa with intestinal metaplasia. Immunohistochemistry showed reactivity to P450 reductase in metaplastic epithelial cells and in pyloric gland cells in glands showing intestinal metaplasia. These cells exhibit NADPH-diaphorase activity. Reverse transcription-PCR analysis and Western blotting showed that CYP1A1 and CYP1A2 were expressed in specimens with intestinal metaplasia. Tissue distribution of CYP1A1 coincided with that of P450 reductase. However, immunoreactivity to CYP1A2 protein was localized only in the pyloric gland cells near the intestinal metaplastic gland. Salmonella typhimurium mutagen assay definitively revealed that microsomes prepared from gastric mucosa with intestinal metaplasia, in particular in the pyloric gland, functionally activated benzo(a)pyrene and 2-amino-3-methylimidazo [4,5-f]quinoline. These results indicate that carcinogen activation by CYP enzymes expressed in the gastric mucosa may contribute to carcinogenesis of the stomach.
M Tatemichi; S Nomura; T Ogura; H Sone; H Nagata; H Esumi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer research     Volume:  59     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-09-16     Completed Date:  1999-09-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3893-8     Citation Subset:  IM    
Investigative Treatment Division, National Cancer Center Research Institute East, Chiba, Japan.
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MeSH Terms
Carcinogens, Environmental / metabolism*,  toxicity
Cytochrome P-450 CYP1A1 / metabolism*
Cytochrome P-450 CYP1A2 / metabolism*
Enzyme Activation
Gastric Mucosa / metabolism,  pathology*
Microsomes / metabolism*,  pathology
Stomach Neoplasms / etiology,  metabolism
Reg. No./Substance:
0/Carcinogens, Environmental; EC P-450 CYP1A1; EC P-450 CYP1A2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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