Document Detail


Muscle-derived stem cell sheets support pump function and prevent cardiac arrhythmias in a model of chronic myocardial infarction.
MedLine Citation:
PMID:  23319053     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Direct intracardiac cell injection for heart repair is hindered by numerous limitations including: cell death, poor spreading of the injected cells, arrhythmia, needle injury, etc. Tissue-engineered cell sheet implantation has the potential to overcome some of these limitations. We evaluated whether the transplantation of a muscle-derived stem cell (MDSC) sheet could improve the regenerative capacity of MDSCs in a chronic model of myocardial infarction. MDSC sheet-implanted mice displayed a reduction in left ventricle (LV) dilation and sustained LV contraction compared with the other groups. The MDSC sheet formed aligned myotubes and produced a significant increase in capillary density and a reduction of myocardial fibrosis compared with the other groups. Hearts transplanted with the MDSC sheets did not display any significant arrhythmias and the donor MDSC survival rate was higher than the direct myocardial MDSC injection group. MDSC sheet implantation yielded better functional recovery of chronic infarcted myocardium without any significant arrhythmic events compared with direct MDSC injection, suggesting this cell sheet delivery system could significantly improve the myocardial regenerative potential of the MDSCs.
Authors:
Naosumi Sekiya; Kimimasa Tobita; Sarah Beckman; Masaho Okada; Burhan Gharaibeh; Yoshiki Sawa; Robert L Kormos; Johnny Huard
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-01-15
Journal Detail:
Title:  Molecular therapy : the journal of the American Society of Gene Therapy     Volume:  21     ISSN:  1525-0024     ISO Abbreviation:  Mol. Ther.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-01     Completed Date:  2013-10-22     Revised Date:  2014-03-06    
Medline Journal Info:
Nlm Unique ID:  100890581     Medline TA:  Mol Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  662-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Arrhythmias, Cardiac / physiopathology,  prevention & control*
Capillaries / physiopathology
Male
Mice
Mice, Inbred NOD
Muscle Cells / cytology*
Myoblasts / cytology,  metabolism,  transplantation
Myocardial Infarction / physiopathology*,  therapy
Myocardium / cytology
Stem Cell Transplantation*
Stem Cells / cytology*
Tissue Engineering*
Vascular Endothelial Growth Factor A / genetics,  metabolism
Ventricular Function, Left
Grant Support
ID/Acronym/Agency:
R21 HL0944402/HL/NHLBI NIH HHS; U54 AR050733/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Vascular Endothelial Growth Factor A
Comments/Corrections

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