Document Detail

Muscle blood flow in cats: comparison of microdialysis ethanol technique with direct measurement.
MedLine Citation:
PMID:  7592229     Owner:  NLM     Status:  MEDLINE    
A quantitative validation of the microdialysis ethanol technique was performed in cat gastrocnemius muscle. Six to eight microdialysis probes were inserted into the isolated muscle preparation and perfused (0.5-10.0 microliters/min) with Krebs-Henseleit buffer containing between 5 and 1,000 mmol/l ethanol. Skeletal muscle blood flow was held constant in the range of 4-99 ml.100 g-1.min-1 by a servo-controlled roller pump and was determined with the microdialysis ethanol technique as well as by timed collection of venous outflow. The ethanol concentration outflow-to-inflow ratio ([ethanol]collected dialysate/[ethanol]infused perfusion medium) decreased in a nonlinear fashion when microdialysis perfusion flow rates of 0.5 and 1.0 microliter/min were employed. However, a linear decrease was found between 4 and approximately 45 ml.100 g-1.min-1 (r = -0.92 to -0.99). The lower outflow-to-inflow ratio was at 4 ml.100 g-1.min-1 (i.e., due to a low probe perfusion flow rate or a large dialysis membrane), the greater the sensitivity of the method was. It is concluded that this nonradioactive technique provides a simple and valid method for determining nutritive blood flow in skeletal muscle.
R C Hickner; U Ekelund; S Mellander; U Ungerstedt; J Henriksson
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  79     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1995-12-18     Completed Date:  1995-12-18     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  638-47     Citation Subset:  IM    
Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden.
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MeSH Terms
Ethanol / diagnostic use*
Microdialysis / instrumentation,  methods*
Muscle, Skeletal / blood supply*
Regional Blood Flow / physiology
Regression Analysis
Reg. No./Substance:

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