Document Detail

Muscle beta-actinin and serum albumin of the chicken are indistinguishable by physicochemical and immunological criteria.
MedLine Citation:
PMID:  6787597     Owner:  NLM     Status:  MEDLINE    
Chicken muscle beta-actinin is considered to be one of the "true" myofibrillar components due to its specific binding to isolated myofibrils. Surprisingly, the direct comparison of this muscle protein with serum albumin, both isolated from chicken, showed that they behaved identically under several electrophoretic conditions. Furthermore, immunoreplica gels and double-immunodiffusion tests with antibodies prepared against beta-actinin established the serological identity of both proteins. No significant differences were found by circular dichroic spectroscopy or in amino acid composition. In addition, the amino-terminal sequences of both proteins were identical (H2N-Asp-Ala-Glu-His-Lys-Ser-Glu-Ile-Ala-His-Arg-Tyr-Asn-Asp-Leu-). Combined, these results strongly indicate that muscle beta-actinin and serum albumin are similar, if not identical.
C W Heizman; G Müller; E Jenny; K J Wilson; F Landon; A Olomucki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  78     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1981 Jan 
Date Detail:
Created Date:  1981-08-20     Completed Date:  1981-08-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  74-7     Citation Subset:  IM    
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MeSH Terms
Actinin / immunology,  isolation & purification*
Actins / metabolism
Amino Acid Sequence
Electrophoresis, Polyacrylamide Gel
Muscle Proteins / isolation & purification*
Myofibrils / analysis
Protein Conformation
Serum Albumin / immunology,  isolation & purification*
Reg. No./Substance:
0/Actins; 0/Muscle Proteins; 0/Serum Albumin; 11003-00-2/Actinin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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