| Muscle amino acid pattern in obese rats. | |
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MedLine Citation:
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PMID: 15481771 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine how the ability of skeletal muscle to manage amino acids is conditioned by obesity. The test was performed in two different models of obese rats: diet-obese rats and genetically obese rats. SUBJECTS: Lean and genetically obese (fa/fa) male Zucker rats were used. DESIGN: For up to 60 d of life lean animals were fed with standard chow pellet or with a hypercaloric cafeteria diet. Genetically obese rats were fed with standard chow pellet during the same period. MEASUREMENTS: Amino acid concentration in the femoral artery and vein and leg blood flow were measured. Free amino acid concentration, protein and nitrogen content and enzyme activities were determined in whole leg muscle. RESULTS: Amino acid availability was increased in diet-obese animals and remained unchanged in the genetically obese group. The genetically obese groups had a lower protein content and unchanged concentration of amino acids in leg muscle. Furthermore, total nitrogen remained unchanged in these animals and they showed an increased activity in alanine aminotransferase and glutamine synthetase. The leg muscle of the diet-obese rats took up several amino acids (Ala, Arg and Val) or released others (mainly Gln and Gly). Conversely, genetically obese rats took up many amino acids and did not release any. CONCLUSIONS: We conclude that in nutritionally obese rats there is an increased availability of amino acids in skeletal muscle. This augmented availability propitiates the increase in the uptake of many amino acids. In genetically obese rats, the lack of variation in amino acid availability points to a possible hereditary alteration that increases the capacity of different amino acid transport systems. Furthermore, the diminished protein content of fa/fa muscle is not due to a lower availability and intracellular pool of amino acids. |
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Authors:
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M C Herrero; X Remesar; C Bladé; L Arola |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity Volume: 21 ISSN: 0307-0565 ISO Abbreviation: Int. J. Obes. Relat. Metab. Disord. Publication Date: 1997 Aug |
Date Detail:
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Created Date: 2004-10-14 Completed Date: 2005-02-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9313169 Medline TA: Int J Obes Relat Metab Disord Country: England |
Other Details:
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Languages: eng Pagination: 698-703 Citation Subset: IM |
Affiliation:
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Department de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, Tarragona, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism* Animals Biological Availability Biological Transport Hindlimb / blood supply Male Muscle, Skeletal / metabolism* Obesity / genetics, metabolism* Overnutrition / metabolism* Rats Rats, Zucker Regional Blood Flow |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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