| Muscle protein synthesis and gene expression during recovery from aerobic exercise in the fasted and fed states. | |
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MedLine Citation:
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PMID: 20720176 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of this investigation was to assess mixed-muscle fractional synthesis rate (FSR) and the expression of genes involved in skeletal muscle remodeling after aerobic exercise in the fasted and fed states. Eight recreationally active males (25 ± 1 yr; Vo(2 max): 52 ± 2 ml·kg(-1)·min(-1)) performed 60-min of cycle ergometry at 72 ± 1% Vo(2 max) on two occasions in a counter-balanced design. Subjects ingested a noncaloric placebo (EX-FAST) or a beverage containing (per kg body wt): 5 kcal, 0.83 g carbohydrate, 0.37 g protein, and 0.03 g fat (EX-FED) immediately and 1 h after exercise. FSR was assessed at rest and following exercise with the use of a l-[ring (2)H(5)]-phenylalanine infusion combined with muscle biopsies at 2 and 6 h postexercise. mRNA expression was assessed at 2 and 6 h postexercise via real-time RT-PCR. FSR was higher (P < 0.05) after exercise in both EX-FAST (0.112 ± 0.010%·h(-1)) and EX-FED (0.129 ± 0.014%·h(-1)) compared with rest (0.071 ± 0.005%·h(-1)). Feeding attenuated the mRNA expression (P < 0.05) of proteolytic factors MuRF-1 (6 h) and calpain-2 (2 and 6 h) postexercise but did not alter FOXO3A, calpain-1, caspase3, or myostatin mRNA expression compared with EX-FAST. Myogenic regulatory factor (MRF4) mRNA was also attenuated (P < 0.05) at 2 and 6 h postexercise in EX-FED compared with EX-FAST. These data demonstrate that a nonexhaustive bout of aerobic exercise stimulates skeletal muscle FSR in the fasted state and that feeding does not measurably enhance FSR between 2 and 6 h after aerobic exercise. Additionally, postexercise nutrient intake attenuates the expression of factors involved in the ubiquitin-proteosome and Ca(2+)-dependent protein degradation pathways. These data provide insight into the role of feeding on muscle protein metabolism during recovery from aerobic exercise. |
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Authors:
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Matthew P Harber; Adam R Konopka; Bozena Jemiolo; Scott W Trappe; Todd A Trappe; Paul T Reidy |
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Publication Detail:
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Type: Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-18 |
Journal Detail:
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Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: 299 ISSN: 1522-1490 ISO Abbreviation: Am. J. Physiol. Regul. Integr. Comp. Physiol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-03 Completed Date: 2010-12-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901230 Medline TA: Am J Physiol Regul Integr Comp Physiol Country: United States |
Other Details:
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Languages: eng Pagination: R1254-62 Citation Subset: IM |
Affiliation:
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Human Performance Laboratory, Ball State Univ., Muncie, IN 47306, USA. mharber@bsu.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Beverages Biopsy Blood Glucose / metabolism Dietary Carbohydrates / administration & dosage Dietary Fats / administration & dosage Dietary Proteins / administration & dosage Eating* Exercise* Fasting* Fatty Acids / blood Gene Expression Regulation Glycogen / metabolism Humans Infusions, Intravenous Insulin / blood Male Muscle Contraction* Muscle Proteins / biosynthesis*, genetics Muscle, Skeletal / metabolism* Oxygen Consumption Phenylalanine / administration & dosage, analogs & derivatives RNA, Messenger / metabolism Recovery of Function Time Factors Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/Dietary Carbohydrates; 0/Dietary Fats; 0/Dietary Proteins; 0/Fatty Acids; 0/Muscle Proteins; 0/RNA, Messenger; 11061-68-0/Insulin; 63-91-2/Phenylalanine; 9005-79-2/Glycogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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