Document Detail


Muscarinic receptor agonists protect cultured bovine trabecular meshwork cells against apoptosis induced by dexamethasone.
MedLine Citation:
PMID:  15124534     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To study whether muscarinic receptor agonists can protect cultured bovine trabecular meshwork cells against apoptosis induced by dexamethasone. METHODS: The third to fifth passages of bovine trabecular meshwork cells were grown to confluence and incubated for 1-14 days in growth media with dexamethasone or pretreatment of pilocarpine or carbachol. The cultures were evaluated for apoptosis by phase-contrast microscopy, fluorescence microscopy, DNA laddering and flow cytometric analysis. RESULTS: Dexamethasone (0.24-0.96 mmol.L-1) induced apoptosis of trabecular meshwork cells in a dose and time-dependent manner. Before 0.48 mmol.L-1 dexamethasone-treatment, 1.84 mmol.L-1 of pilocarpine or 2.74 mmol.L-1 of carbachol added could significantly reduce apoptotic percentage. CONCLUSION: Muscarinic receptor agonists can protect cultured bovine trabecular meshwork cells against apoptosis induced by dexamethasone.
Authors:
Yajuan Gu; Shujun Zeng; PengXin Qiu; Yuping Wu; Dawei Peng; Guangmei Yan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu     Volume:  20     ISSN:  1000-4432     ISO Abbreviation:  Yan Ke Xue Bao     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-05-04     Completed Date:  2004-07-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8605666     Medline TA:  Yan Ke Xue Bao     Country:  China    
Other Details:
Languages:  eng     Pagination:  42-7     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510089, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*
Carbachol / pharmacology
Cattle
Cells, Cultured
Dexamethasone / antagonists & inhibitors
Flow Cytometry
Muscarinic Agonists / pharmacology*
Pilocarpine / pharmacology
Trabecular Meshwork / cytology*
Chemical
Reg. No./Substance:
0/Muscarinic Agonists; 50-02-2/Dexamethasone; 51-83-2/Carbachol; 92-13-7/Pilocarpine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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