Document Detail

Murine pulmonary macrophages: evaluation of lung lavage fluids, miniaturized monolayers, and candidacidal activity.
MedLine Citation:
PMID:  6336925     Owner:  NLM     Status:  MEDLINE    
Optimal yields of pulmonary macrophages (PuM) from lavage of normal murine lungs have been obtained by the study of several different lung lavage fluids. Approximately 1 X 10(6) PuM can be consistently recovered from mice weighing 20 to 30 g, using 15 ml of phosphate buffered saline with 0.1% EDTA, with or without 10% fetal calf serum at 37 degrees C. Use of Terasaki tray wells for monolayer formation has allowed a relatively small number of cells (75,000) to be used to prepare multiple replicate wells from each mouse. Simultaneous functional comparisons with other cell types, e.g., peritoneal macrophages (PeM) were performed using this practical and economical system. Data on the phagocytosis and killing of Candida albicans by PuM and PeM are presented as evidence of the utility of this miniaturized system.
A M Sugar; E Brummer; D A Stevens
Related Documents :
19344975 - 14-kda phosphohistidine phosphatase and its role in human lung cancer cell migration an...
10460005 - Low-grade b-cell lymphoma of mucosa-associated lymphoid tissue in the lung: a report of...
20195465 - Differentiation, distribution and gammadelta t cell-driven regulation of il-22-producin...
9533935 - Cyclooxygenase isoenzyme localization and mrna expression in rat lungs.
17209045 - Ras/erk1/2-mediated stat3 ser727 phosphorylation by familial medullary thyroid carcinom...
9156045 - The basal rate of cell proliferation in normal human parathyroid tissue: implications f...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American review of respiratory disease     Volume:  127     ISSN:  0003-0805     ISO Abbreviation:  Am. Rev. Respir. Dis.     Publication Date:  1983 Jan 
Date Detail:
Created Date:  1983-02-25     Completed Date:  1983-02-25     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0370523     Medline TA:  Am Rev Respir Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  110-2     Citation Subset:  AIM; IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Candida albicans / immunology*
Cells, Cultured
Lung / cytology,  immunology*
Macrophages / immunology*
Mice, Inbred BALB C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Estimation of total lung capacity by planimetry of chest radiographs in children 5 to 10 years of ag...
Next Document:  Anti-intercellular substance antibodies. Presence in serum samples of 14 patients without pemphigus.