Document Detail


Murine pulmonary macrophages: evaluation of lung lavage fluids, miniaturized monolayers, and candidacidal activity.
MedLine Citation:
PMID:  6336925     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Optimal yields of pulmonary macrophages (PuM) from lavage of normal murine lungs have been obtained by the study of several different lung lavage fluids. Approximately 1 X 10(6) PuM can be consistently recovered from mice weighing 20 to 30 g, using 15 ml of phosphate buffered saline with 0.1% EDTA, with or without 10% fetal calf serum at 37 degrees C. Use of Terasaki tray wells for monolayer formation has allowed a relatively small number of cells (75,000) to be used to prepare multiple replicate wells from each mouse. Simultaneous functional comparisons with other cell types, e.g., peritoneal macrophages (PeM) were performed using this practical and economical system. Data on the phagocytosis and killing of Candida albicans by PuM and PeM are presented as evidence of the utility of this miniaturized system.
Authors:
A M Sugar; E Brummer; D A Stevens
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American review of respiratory disease     Volume:  127     ISSN:  0003-0805     ISO Abbreviation:  Am. Rev. Respir. Dis.     Publication Date:  1983 Jan 
Date Detail:
Created Date:  1983-02-25     Completed Date:  1983-02-25     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0370523     Medline TA:  Am Rev Respir Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  110-2     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Candida albicans / immunology*
Cells, Cultured
Irrigation
Lung / cytology,  immunology*
Macrophages / immunology*
Male
Methods
Mice
Mice, Inbred BALB C
Phagocytosis*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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