Document Detail


Murine norovirus-1 entry into permissive macrophages and dendritic cells is pH-independent.
MedLine Citation:
PMID:  19463729     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Murine norovirus (MNV) is a recently discovered mouse pathogen. Unlike the fastidious human noroviruses that cause the overwhelming majority of non-bacterial gastroenteritis worldwide, MNV readily infects cells in culture. Its replication in primary murine macrophages and dendritic cells and their derived cell lines allows the study of norovirus cell entry for the first time. In this study we determined the role of pH during MNV-1 infection since the low pH environment of endosomes often triggers uncoating of viruses. We demonstrated that MNV-1 viral titers by plaque assay and expression of the non-structural protein VPg by immunofluorescence were not affected by pH in cultured and primary macrophages and dendritic cells in the presence of two known endosome acidification inhibitors, bafilomycin A1 and chloroquine. These data indicate that MNV-1 enters permissive cells in a pH-independent manner.
Authors:
Jeffrey W Perry; Stefan Taube; Christiane E Wobus
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-03-14
Journal Detail:
Title:  Virus research     Volume:  143     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-05-25     Completed Date:  2009-07-21     Revised Date:  2014-09-24    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  125-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antirheumatic Agents / pharmacology
Caliciviridae Infections / drug therapy*,  metabolism,  virology
Cell Culture Techniques
Chloroquine / pharmacology
Dendritic Cells / chemistry,  drug effects,  virology*
Endosomes / chemistry,  drug effects
Enzyme Inhibitors / pharmacology
Hydrogen-Ion Concentration / drug effects
Macrolides / pharmacology
Macrophages / chemistry,  drug effects,  virology*
Male
Mice
Norovirus / drug effects,  metabolism,  physiology*
Viral Nonstructural Proteins / biosynthesis
Virus Internalization / drug effects*
Grant Support
ID/Acronym/Agency:
1-U54-AI-057153/AI/NIAID NIH HHS; T32 GM 07544/GM/NIGMS NIH HHS; U54 AI057153/AI/NIAID NIH HHS; U54 AI057153-05S10011/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antirheumatic Agents; 0/Enzyme Inhibitors; 0/Macrolides; 0/Viral Nonstructural Proteins; 886U3H6UFF/Chloroquine; 88899-55-2/bafilomycin A1
Comments/Corrections

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