Document Detail


Murine A-myb: evidence for differential splicing and tissue-specific expression.
MedLine Citation:
PMID:  8084617     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The myb gene family consists of three members, A-myb, B-myb and c-myb. The proteins encoded by these genes bind DNA in a sequence-specific manner and regulate transcription of target genes. In this communication, we report the nucleotide sequence of murine A-myb cDNA. This gene encodes a protein of 751 amino acids with an estimated molecular weight of 83 kDa. cDNA sequence analysis of multiple independent cDNA clones reveals the presence of alternatively spliced mRNAs that encode smaller proteins. Northern blot analysis of adult mouse tissue RNAs reveals A-myb expression predominantly in the testis, with very low levels of expression in the ovaries, spleen and brain. In situ hybridization analysis of adult mouse testis shows that this gene is expressed at high levels in type A spermatogonia (stem cells), and preleptotene and pachytene spermatocytes, with concomitant down-regulation of expression upon terminal differentiation of these cells into mature spermatozoa. This pattern of expression suggests that A-myb is involved in the control of proliferation and differentiation of spermatogonia. This potential function of A-myb in spermatogenesis is reminiscent of the role of c-myb in hematopoiesis.
Authors:
R V Mettus; J Litvin; A Wali; A Toscani; K Latham; K Hatton; E P Reddy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Oncogene     Volume:  9     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1994-10-13     Completed Date:  1994-10-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  3077-86     Citation Subset:  IM    
Affiliation:
Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA 19140.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/L35261
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Cloning, Molecular
DNA, Complementary
Down-Regulation
Humans
Male
Mice
Molecular Sequence Data
Oncogenes*
Proto-Oncogene Proteins / biosynthesis,  genetics*
RNA Splicing*
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Spermatogenesis / genetics
Tissue Distribution
Trans-Activators / biosynthesis,  genetics*
Grant Support
ID/Acronym/Agency:
5-P30-CA12227/CA/NCI NIH HHS; CA-52009/CA/NCI NIH HHS; GM49489/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/MYBL1 protein, human; 0/Mybl1 protein, mouse; 0/Proto-Oncogene Proteins; 0/Trans-Activators

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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