Document Detail

Murine mast cells secrete a unique profile of cytokines and prostaglandins in response to distinct TLR2 ligands.
MedLine Citation:
PMID:  19382314     Owner:  NLM     Status:  MEDLINE    
Mast cells (MCs) are important effector cells in host defense against bacteria. In the course of a bacterial infection, MCs can be activated by various mechanisms, i.e. bacterial toxins, endogenously produced infection-associated peptides or via complement receptors, fimbrial adhesion molecules and toll-like receptors (TLRs). While some of these mechanisms are well established, the effects of TLR2 ligand-driven MC activation are far less understood. Here, we show that murine mature connective tissue-type MCs, but not immature bone marrow-derived cultured mast cells, express significant amounts of full length TLR2 on their surface. Activation by various TLR2 ligands only induces the selective release of cytokines in peritoneum-derived cultured mast cells (PCMCs) with preferential secretion of pro-inflammatory cytokines (IL-6 > IL-17 > IFN-gamma TNF > IL-1 > GM-CSF) upon stimulation with lipoteichoic acid (LTA). This response is much lower in PCMCs stimulated with the TLR2/6 agonist macrophage-activating lipopeptide-2 (MALP-2), which most prominently triggers the release of the immunomodulatory cytokine IL-10. Furthermore, only LTA but not MALP-2 induces prostaglandin D2 secretion which is again restricted to the mature MC phenotype. These findings suggest that TLR2 ligand-mediated activation of mature MCs, i.e. tissue-residing cells, which most likely occurs during infection, can selectively raise a potent inflammatory or anti-inflammatory response, depending on TLRs which are engaged.
Salima Mrabet-Dahbi; Martin Metz; Anne Dudeck; Torsten Zuberbier; Marcus Maurer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental dermatology     Volume:  18     ISSN:  1600-0625     ISO Abbreviation:  Exp. Dermatol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-20     Completed Date:  2009-08-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9301549     Medline TA:  Exp Dermatol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  437-44     Citation Subset:  IM    
Allergie-Centrum-Charité, Department of Dermatology and Allergy, Charité- Universitätsmedizin Berlin, Berlin, Germany.
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MeSH Terms
Bone Marrow Cells / cytology
Cell Separation
Cytokines / biosynthesis*
Flow Cytometry
Lipopeptides / biosynthesis
Lipopolysaccharides / pharmacology
Mast Cells / cytology*
Mice, Inbred C57BL
Prostaglandin D2 / secretion
Prostaglandins / biosynthesis*
Teichoic Acids / pharmacology
Toll-Like Receptor 2 / biosynthesis*
Reg. No./Substance:
0/Cytokines; 0/Ligands; 0/Lipopeptides; 0/Lipopolysaccharides; 0/Prostaglandins; 0/Teichoic Acids; 0/Tlr2 protein, mouse; 0/Toll-Like Receptor 2; 0/macrophage stimulatory lipopeptide 2; 41598-07-6/Prostaglandin D2; 56411-57-5/lipoteichoic acid

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