Document Detail

Murine glomerular mesangial cell uptake of apoptotic cells is inefficient and involves serum-mediated but complement-independent mechanisms.
MedLine Citation:
PMID:  12452836     Owner:  NLM     Status:  MEDLINE    
An increased number of apoptotic bodies have been detected in glomeruli of non-nephritic kidneys of C1q-deficient mice. In these mice an in vivo impaired uptake of apoptotic cells by peritoneal macrophages was also demonstrated. Here we investigated whether C1q plays a role in the in vitro clearance of apoptotic cells by glomerular mesangial cells. Phagocytosis was assessed using a novel flow cytometric assay that was validated by immunofluorescence studies. The uptake of apoptotic cells by mesangial cells, measured as percentage of mesangial cells ingesting apoptotic cells, was approximately 25%, 10% and 10% for a T cell lymphoma line (RMA), thymocytes and neutrophils, respectively. The uptake reached a plateau phase after 3 h, was specific for apoptotic cells and was mediated by serum but not by complement components C1q or C3. The phagocytosis of apoptotic cells was significantly inhibited by Arg-Gly-Asp-Ser (RGDS), a peptide capable of blocking the interaction of thrombospondin with CD36 or the vitronectin receptor. Pretreatment of the mesangial cells with dexamethasone (200 nm) but not with LPS increased the uptake markedly. These findings indicate that murine mesangial cells are capable of taking up syngeneic apoptotic cells, although much less efficiently than professional phagocytic cells. They also show that serum proteins other than complement components mediate the removal of apoptotic cells by murine mesangial cells in vitro.
J Cortes-Hernandez; L Fossati-Jimack; A Carugati; P K Potter; M J Walport; H T Cook; M Botto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  130     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-27     Completed Date:  2003-03-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  459-66     Citation Subset:  IM    
Rheumatology Section and Histopathology Department, Division of Medicine, Faculty of Medicine, Imperial College, Hammersmith Campus, London, UK.
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MeSH Terms
Blood Proteins / metabolism*
Complement C1q*
Complement C3
Dexamethasone / pharmacology
Flow Cytometry
Glomerular Mesangium / cytology*,  drug effects
Glucocorticoids / pharmacology
Mice, Inbred C57BL
Mice, Knockout
Oligopeptides / pharmacology
Stimulation, Chemical
Tumor Cells, Cultured
Reg. No./Substance:
0/Blood Proteins; 0/Complement C3; 0/Glucocorticoids; 0/Oligopeptides; 50-02-2/Dexamethasone; 80295-33-6/Complement C1q; 91037-65-9/arginyl-glycyl-aspartyl-serine

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