Document Detail


Murine bone marrow-derived mast cells exhibit evidence of both apoptosis and oncosis after IL-3 deprivation.
MedLine Citation:
PMID:  10709846     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
IL-3 deprivation has been reported to induce apoptosis of bone marrow-derived mast cells. In order to evaluate this type of cell death further, we employed trypan blue and propidium iodide stainings, photometric enzyme immunoassay, fluorescence measurement of caspase-3, DNA electrophoresis, flow cytometry and transmission electron microscopy. In this experiment, although several evidences supporting apoptosis were demonstrated some findings were not consistent with typical apoptosis. On the other hand, electron microscopical observation demonstrated that most cells from all the time phases after IL-3 deprivation showed the morphology of typical oncosis, i.e. cell swelling, disintegration of ultrastructure and subsequent karyolysis. Only a small number of cells from the later time phases showed apoptotic morphology. We here suggest that BMMCs undergo both apoptosis and oncosis after IL-3 deprivation and that the dominant type of prelethal change is oncosis in all time phases, although apoptosis also plays a partial role in the late time phases.
Authors:
B S Park; G C Kim; S J Back; N D Kim; Y S Kim; S K Kim; M H Jeong; Y J Lim; Y H Yoo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Immunological investigations     Volume:  29     ISSN:  0882-0139     ISO Abbreviation:  Immunol. Invest.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-03-24     Completed Date:  2000-03-24     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8504629     Medline TA:  Immunol Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  51-60     Citation Subset:  IM    
Affiliation:
Department of Oral Anatomy and Cell Biology, Pusan National University College of Dentistry, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis*
Bone Marrow Cells / cytology*,  metabolism*,  ultrastructure
Cell Death
Cells, Cultured
Interleukin-3 / metabolism*
Mast Cells / cytology*,  metabolism*,  ultrastructure
Mice
Mice, Inbred BALB C
Chemical
Reg. No./Substance:
0/Interleukin-3

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