Document Detail


Murine trophoblast cells induce NK cell interferon-gamma production through KLRK1.
MedLine Citation:
PMID:  20484740     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Murine models suggest that natural killer (NK) cells are important for normal implantation site development, in part, through the production of interferon gamma (IFNG). As KLRK1 (NKG2D) is expressed on human and murine uterine NK (uNK) cells, we examined the role of KLRK1 in the interaction between murine trophoblasts and NK cells. Flow cytometric analysis revealed that both murine trophoblast stem (TS) cells and differentiated trophoblast giant cells expressed the KLRK1 ligand retinoic acid early transcript 1, or RAET1. Coculture of activated NK cells with either TS cells or giant cells led to the production of IFNG, as measured by ELISA. In addition, coculture with TS cells led to the downregulation of KLRK1. Both responses were inhibited by soluble KLRK1 ligand, but not by irrelevant protein. Further studies demonstrated the presence of KLRK1 ligand on uterine cells derived from either virgin or pregnant mice, although uterine RAET1 protein expression was upregulated in vitro by progesterone, but not estradiol. We suggest that the interaction of KLRK1 and RAET1 may be involved in IFNG production by uNK cells, and thus, this receptor-ligand pair may contribute to successful murine implantation site development.
Authors:
Leonidas N Carayannopoulos; Jennifer L Barks; Wayne M Yokoyama; Joan K Riley
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-05-19
Journal Detail:
Title:  Biology of reproduction     Volume:  83     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-23     Completed Date:  2010-11-30     Revised Date:  2011-09-13    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  404-14     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Cells, Cultured
Coculture Techniques
Enzyme-Linked Immunosorbent Assay
Female
Flow Cytometry
Interferon-gamma / biosynthesis*,  immunology
Killer Cells, Natural / immunology,  metabolism*
Membrane Proteins / metabolism
Mice
NK Cell Lectin-Like Receptor Subfamily K / immunology,  metabolism*
Pregnancy
Reverse Transcriptase Polymerase Chain Reaction
Stromal Cells / immunology,  metabolism
Trophoblasts / immunology,  metabolism*
Grant Support
ID/Acronym/Agency:
5K12HD001459/HD/NICHD NIH HHS; K08AI57361/AI/NIAID NIH HHS; //Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Klrk1 protein, mouse; 0/Membrane Proteins; 0/NK Cell Lectin-Like Receptor Subfamily K; 0/Raet1a protein, mouse; 82115-62-6/Interferon-gamma
Comments/Corrections

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