| Mureinolytic ability of the rumen ciliate Diploplastron affine. | |
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MedLine Citation:
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PMID: 20680560 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rumen ciliate protozoa intensively engulf bacteria. However, their ability to utilize murein which is the main polysaccharide of bacterial cell wall has hardly been recognized. The present study concerns the ability of the rumen protozoa Diploplastron affine to digest and ferment murein. The ciliates were isolated from the rumen fluid and grown in vitro or inoculated into the rumen of defaunated sheep. The results of long-term cultivation of protozoa showed a positive correlation between their number and murein content in the culture medium. It was also found that bacteria-free D. affine ciliates incubated with or without murein produced volatile fatty acids at the rate of 12.3 and 8.7 pmol/h per protozoan, respectively, acetic, butyric and propionic acids being the three main acids released to the medium. Enzyme studies performed with the use of protozoan cell extract prepared from bacteria-free ciliates degraded murein at a rate of 25 U/mg protein per h; two mureinolytic enzymes were identified by zymographic technique in the examined preparation. |
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Authors:
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G Bełzecki; R Miltko; E Kwiatkowska; T Michałowski |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-03 |
Journal Detail:
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Title: Folia microbiologica Volume: 55 ISSN: 1874-9356 ISO Abbreviation: Folia Microbiol. (Praha) Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-08-03 Completed Date: 2010-11-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376757 Medline TA: Folia Microbiol (Praha) Country: United States |
Other Details:
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Languages: eng Pagination: 312-4 Citation Subset: IM |
Affiliation:
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The Kielanowski Institute of Animal Physiology and Nutrition Polish Academy of Sciences, 05-110, Jabłonna, Poland. g.belzecki@ifzz.pan.pl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Ciliophora / isolation & purification, metabolism* Culture Media / chemistry Fatty Acids, Volatile / metabolism Peptidoglycan / metabolism* Rumen / parasitology* Sheep |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Fatty Acids, Volatile; 0/Peptidoglycan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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