Document Detail

Murein biosynthesis in ether permeabilized Escherichia coli starting from early peptidoglycan precursors.
MedLine Citation:
PMID:  7036928     Owner:  NLM     Status:  MEDLINE    
ETB, ether treated bacteria, from E. coli and other Gram-negative strains, contain in a cell-free system all enzymes necessary for murein biosynthesis. Starting with a variety of combinations of peptidoglycan precursors, high yields of sodium dodecylsulfate (SDS, 4%) insoluble murein or murein like material were synthesized. The amount of newly synthesized SDS insoluble material (NSM) was dependent upon the growing phase at which cells had been harvested for preparation of ETB. This data may provide some insight into the regulation of peptidoglycan biosynthesis. Starting from early peptidoglycan precursors, the cell-free synthesis of NSM was inhibited by specific inhibitors of murein synthesis, such as D-cycloserine, D-fluoroalanine, 2-amino-ethylphosphonate, analogues of D-alanyl-D-alanine and beta-lactam antibiotics at appropriate concentrations. Some D-alanyl-D-alanine analogues and 4-chlorodiaminopimelic acid were incorporated into NSM in place of their corresponding natural substrates.
D Maass; H Pelzer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives of microbiology     Volume:  130     ISSN:  0302-8933     ISO Abbreviation:  Arch. Microbiol.     Publication Date:  1981 Dec 
Date Detail:
Created Date:  1982-04-12     Completed Date:  1982-04-12     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0410427     Medline TA:  Arch Microbiol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  301-6     Citation Subset:  IM    
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MeSH Terms
Cell Membrane Permeability / drug effects
Escherichia coli / enzymology,  metabolism*
Ether, Ethyl / pharmacology
Oligopeptides / metabolism
Peptidoglycan / biosynthesis*,  metabolism
Uridine Diphosphate N-Acetylglucosamine / metabolism
Uridine Diphosphate N-Acetylmuramic Acid / analogs & derivatives,  metabolism
Reg. No./Substance:
0/Oligopeptides; 0/Peptidoglycan; 0/Uridine Diphosphate N-Acetylmuramic Acid; 16124-22-4/UDP-N-acetylmuramic acid pentapeptide; 528-04-1/Uridine Diphosphate N-Acetylglucosamine; 60-29-7/Ether, Ethyl

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