Document Detail

Multivitamin production in Lactococcus lactis using metabolic engineering.
MedLine Citation:
PMID:  15113564     Owner:  NLM     Status:  MEDLINE    
The dairy starter bacterium Lactococcus lactis has the potential to synthesize both folate (vitamin B11) and riboflavin (vitamin B2). By directed mutagenesis followed by selection and metabolic engineering we have modified two complicated biosynthetic pathways in L. lactis resulting in simultaneous overproduction of both folate and riboflavin: Following exposure to the riboflavin analogue roseoflavin we have isolated a spontaneous mutant of L. lactis strain NZ9000 that was changed from a riboflavin consumer into a riboflavin producer. This mutant contained a single base change in the regulatory region upstream of the riboflavin biosynthetic genes. By the constitutive overproduction of GTP cyclohydrolase I in this riboflavin-producing strain, the production of folate was increased as well. Novel foods, enriched through fermentation using these multivitamin-producing starters, could compensate the B-vitamin-deficiencies that are common even in highly developed countries and could specifically be used in dietary foods for the large fraction of the Caucasian people (10-15%) with mutations in the methylene tetrahydrofolate reductase (MTHFR).
Wilbert Sybesma; Catherine Burgess; Marjo Starrenburg; Douwe van Sinderen; Jeroen Hugenholtz
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Metabolic engineering     Volume:  6     ISSN:  1096-7176     ISO Abbreviation:  Metab. Eng.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-04-28     Completed Date:  2004-11-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815657     Medline TA:  Metab Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  109-15     Citation Subset:  IM    
Department of Flavor, Nutrition and Natural Ingredients, Wageningen Centre for Food Sciences, NIZO food research, Kernhemseweg 2, P.O. Box 20, 6710 BA Ede, The Netherlands.
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MeSH Terms
Folic Acid / biosynthesis*,  genetics*
Genetic Enhancement / methods
Lactococcus lactis / genetics*,  growth & development,  metabolism*
Protein Engineering / methods*
Recombinant Proteins / metabolism
Riboflavin / biosynthesis*,  genetics*
Vitamins / biosynthesis,  genetics
Reg. No./Substance:
0/Recombinant Proteins; 0/Vitamins; 59-30-3/Folic Acid; 83-88-5/Riboflavin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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