Document Detail


Multivariate cluster analysis to study motility activation of Solea senegalensis spermatozoa: a model for marine teleosts.
MedLine Citation:
PMID:  18367506     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Computer-assisted sperm analysis (CASA) and clustering analysis have enabled to study sperm subpopulations in mammals, but their use in fish sperm has been limited. We have used spermatozoa from Senegalese sole (Solea senegalensis) as a model for subpopulation analysis in teleostei using two different activating solutions. Semen from six males was activated using 1100 mOsm/kg solutions: artificial seawater (ASW) or sucrose solution (SUC). Motility was acquired at 15, 30, 45, and 60 s post-activation. CASA parameters were combined into two principal components, which were used in a non-hierarchical clustering analysis, obtaining four subpopulations (CL): CL1 (slow/non-linear), CL2 (slow/linear), CL3 (fast/non-linear), and CL4 (fast/linear). We detected spermatozoa lysis, especially in ASW. Sperm motility was higher for SUC and decreased with time. The subpopulation proportions varied with time and activating treatment, showing both an increase in CL1 and CL2 and a decrease in CL3 and CL4 with time. Both CL3 and CL4 were higher in samples activated with SUC, at least in early post-activation. Proportions of CL3 and CL4 at 15 s were associated with higher quality at 60 s and with lower lysis. A second clustering analysis was conducted, classifying the males accordingly to their motility subpopulations. This analysis showed a high heterogeneity between samples. Subpopulation analysis of CASA data can be applied to Solea spermatozoa, allowing identification of potentially interesting sperm subpopulations. Future studies might benefit from these techniques to establish the relationship of these subpopulations with fish sperm quality and fertility, helping to characterize males according to their reproductive potential.
Authors:
Felipe Martínez-Pastor; Elsa Cabrita; Florbela Soares; Luis Anel; Maria Teresa Dinis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  135     ISSN:  1741-7899     ISO Abbreviation:  Reproduction     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-27     Completed Date:  2008-08-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  England    
Other Details:
Languages:  eng     Pagination:  449-59     Citation Subset:  IM    
Affiliation:
Biology of Reproduction Group, National Wildlife Research Institute (IREC) (CSIC-UCLM-JCCM), 02071 Albacete, Spain Institute for Regional Development (IDR), UCLM, 02071 Albacete, Spain. felipe.martinez@uclm.es
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MeSH Terms
Descriptor/Qualifier:
Animals
Cluster Analysis
Flatfishes / physiology*
Male
Models, Biological
Sperm Motility / physiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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