| Multivariate expression analysis of the gene network underlying sexual development in turtle embryos with temperature-dependent and genotypic sex determination. | |
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MedLine Citation:
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PMID: 20110645 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Sexual development has long been the target of study and despite great advances in our understanding of the composition and regulation of the gene network underlying gonadogenesis, our knowledge remains incomplete. Of particular interest is the relative role that the environment and the genome play in directing gonadal formation, especially the effect of environmental temperature in directing this process in vertebrates. Comparative analyses in closely related taxa with contrasting sex-determining mechanisms should help fill this gap. Here I present a multivariate study of the regulation of the gene network underlying sexual development in turtles with temperature-dependent (TSD; Chrysemys picta) and genotypic sex determination (GSD; Apalone mutica). I combine novel data on SOX9 and DMRT1 from these species with contrasting sex-determining mechanisms for the first time with previously reported data on DAX1, SF-1 (NR5A1), WT1, and aromatase (CYP19A1) from these same taxa. Comparative expression analyses of SOX9 and DMRT1 from these and other species indicate additional elements whose expression has diverged among TSD taxa, further supporting the notion that significant evolutionary changes have accrued in the regulation of the TSD gene network in reptiles. A non-parametric MANOVA revealed that temperature had a significant effect in multivariate gene expression in C. picta that varied during embryonic development, whereas the covariation of gene expression in A. mutica was insensitive to temperature. A phenotypic trajectory analysis (PTA) of gene expression comparing both species directly indicated that the relative covariation in gene expression varied between temperatures in C. picta. Furthermore, the 25 degrees C trajectory of C. picta differed from that of A. mutica in the magnitude of gene expression change. Additional analyses revealed a stronger covariation in gene expression and a more interconnected regulatory network in A. mutica, consistent with the hypothesis that sexual development is a more canalized process in A. mutica, as would be expected if GSD evolved in this lineage through directional selection from its TSD ancestor. |
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Authors:
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N Valenzuela |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-01-22 |
Journal Detail:
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Title: Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation Volume: 4 ISSN: 1661-5433 ISO Abbreviation: Sex Dev Publication Date: 2010 |
Date Detail:
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Created Date: 2010-03-17 Completed Date: 2010-08-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101316472 Medline TA: Sex Dev Country: Switzerland |
Other Details:
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Languages: eng Pagination: 39-49 Citation Subset: IM |
Copyright Information:
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(c) 2010 S. Karger AG, Basel. |
Affiliation:
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Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, Iowa 50011, USA. nvalenzu @ iastate.edu |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/FJ791118; FJ791119 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Body Temperature / physiology* Cloning, Molecular Embryo, Nonmammalian / metabolism* Female Gene Expression Regulation, Developmental Gene Regulatory Networks / genetics* Genotype Male Molecular Sequence Data Multivariate Analysis Principal Component Analysis SOX9 Transcription Factor / chemistry Sex Determination (Genetics)* Sexual Development / genetics* Transcription Factors / chemistry, genetics, metabolism Turtles / embryology*, genetics* |
| Chemical | |
Reg. No./Substance:
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0/DMRT1 protein; 0/SOX9 Transcription Factor; 0/Transcription Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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