Document Detail


Multitrack system for superfusing isolated cardiac myocytes.
MedLine Citation:
PMID:  12531725     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new system for studying mechanical activity of freshly isolated cardiac myocytes from up to four experimental groups simultaneously is described. Suspensions of cardiac myocytes isolated from adult rat hearts were drawn into microhematocrit capillary tubes, which were then mounted in parallel fashion between two four-channel tubing manifolds placed on the movable stage of an inverted microscope. Within a few minutes, cells settled and attached to the bottom of the tubes and then could be superfused with various test solutions. The system allowed for electrical field stimulation, rapid changes in bathing solutions, control of temperature, and simulation of ischemia and reperfusion with measurements of the effects of such interventions on both populations of cells (low power survey) and individual myocytes (high power). Myocyte responses to these various interventions are described. The primary advantage of this system is the ability to conduct experiments on cardiac myocytes isolated concurrently from multiple experimental groups at the same time and under identical conditions.
Authors:
Lois Jane Heller; David E Mohrman; Juline A Smith; Kendall B Wallace
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2003-01-16
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  284     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-07     Completed Date:  2003-05-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H1872-8     Citation Subset:  IM    
Affiliation:
Department of Physiology, School of Medicine, University of Minnesota-Duluth, 1035 University Drive, Duluth, MN 55812, USA. lheller@d.umn.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Caffeine / pharmacology
Electric Stimulation / instrumentation*,  methods
Male
Myocardial Contraction / drug effects,  physiology*
Myocardial Reperfusion Injury / physiopathology*
Myocytes, Cardiac / physiology*
Phosphodiesterase Inhibitors / pharmacology
Rats
Rats, Sprague-Dawley
Temperature
Grant Support
ID/Acronym/Agency:
HL 58016/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Phosphodiesterase Inhibitors; 58-08-2/Caffeine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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